|Detection of Human/Mouse/Rat CARD11/CARMA1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4839) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CARD11/CARMA1 at approximately 133 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
CARMA1 (CARD-MAGUK1; also CARD11) is a 133 kDa member of the MAGUK family of proteins. It is a cytoplasmic promoter of Bcl10 phosphorylation, and as such, regulates the effectiveness of NF-kappa B signaling. Human CARMA1 is 1154 amino acids (aa) in length and contains an N-terminal CARD region, two coiled-coil (CC) domains (aa 123‑250 and 295‑442), and a PZD, SH3 and MAGUK domain (aa 996‑1133). The CC1 and MAGUK domains position the molecule, while CC1 and CC2 mediate a necessary dimerization/oligomerization. There is one potential alternate start site seven aa upstream from the standard start site. Over aa 263-442, human CARMA1 is 98% aa identical to both mouse and canine CARMA1.
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