|Detection of Human/Mouse/Rat CRADD by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4680) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRADD at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
CRADD (Caspase and RIP adapter with death domain; also RAIDD) is a 22 kDa, widely-expressed cytosolic adaptor protein that participates in apoptosis. Mouse CRADD is 199 amino acids (aa) in length. It contains an N-terminal CARD region (aa 1‑92) and a C-terminal DD/death domain (aa 116‑188). The CARD interacts with caspase-2, while the DD recruits either PIDD or RIP. These interactions contribute to the activation of an apoptotic cascade. Mouse CRADD shows two potential additional isoforms. One shows an alternate start site at Met55, while a second shows a 70 aa substitution for aa’s 101‑199. Mouse CRADD shares 90% and 95% aa identity with human and rat CRADD, respectively.
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