Human/Mouse/Rat Cysteine Conjugate beta-Lyase/CCBL1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Cysteine Conjugate  beta -Lyase/CCBL1 by Western Blot.
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Human/Mouse/Rat Cysteine Conjugate beta-Lyase/CCBL1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat Cysteine Conjugate beta -Lyase/CCBL1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cysteine Conjugate beta -Lyase/CCBL1
Accession # Q16773
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Cysteine Conjugate  beta -Lyase/CCBL1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Cysteine Conjugate beta -Lyase/CCBL1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Cysteine Conjugate beta -Lyase/CCBL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cysteine Conjugate beta -Lyase/CCBL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cysteine Conjugate beta-Lyase/CCBL1

CCBL1 (Cysteine Conjugate beta -Lyase 1; also GTK and KAT-I) is a 90-120 kDa homodimeric member of the Class I Pyridoxyl-phosphate-dependent aminotransferase family of enzymes. It is widely expressed, being found in fibroblasts, renal epithelium, hepatocytes, neurons and astrocytes. CCBL1 has multiple activities, including converting prodrugs into active formulations, generating kynurenic acid from kynurenine, thereby antagonizing NMDA receptors (but not likely presynaptic alpha 7 AChRs), and blocking the release of proinflammatory cytokines by microglia via GPCR-35 antagonism. Human CCBL1 is 422 amino acids (aa) in length, and contains one aspartate aminotransferase-like region (aa 31-417). There are three potential splice variants, one that shows a deletion of aa 68-117, a second that shows a deletion of aa 251-422, and a third that contains a 21 aa substitution for aa 230-250. While the 422 aa isoform is considered cytosolic, there is the potential for a 34 aa N-terminal extension that would act as a mitochondrial targeting sequence. Such a sequence is reported for rat and nonhuman primate. Over aa 182-422, human CCBL1 shares 82% aa sequence identity with mouse CCBL1.

Entrez Gene IDs
883 (Human); 70266 (Mouse); 311844 (Rat)
Alternate Names
CCBL1; Cysteine Conjugate betaLyase; Cysteine Conjugate beta-Lyase; cysteine conjugate-beta lyase, cytoplasmic; cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase K; Cysteine-S-conjugate beta-lyase; EC; EC; EC; FLJ95217; Glutamine Transaminase K; glutamine-phenylpyruvate aminotransferase; Glutamine--phenylpyruvate transaminase; GTKMGC29624; KAT1; KATI; KATIbeta-lysase, kidney; Kyneurenine Aminotransferase I; kyneurenine aminotransferase; kyneurenine aminotransferase); Kynurenine aminotransferase I; kynurenine--oxoglutarate transaminase 1; Kynurenine--oxoglutarate transaminase I

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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