Detection of Human, Mouse, and Rat Cysteine Conjugate beta -Lyase/CCBL1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Cysteine Conjugate beta -Lyase/CCBL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cysteine Conjugate beta -Lyase/CCBL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cysteine Conjugate beta-Lyase/CCBL1
CCBL1 (Cysteine Conjugate beta -Lyase 1; also GTK and KAT-I) is a 90-120 kDa homodimeric member of the Class I Pyridoxyl-phosphate-dependent aminotransferase family of enzymes. It is widely expressed, being found in fibroblasts, renal epithelium, hepatocytes, neurons and astrocytes. CCBL1 has multiple activities, including converting prodrugs into active formulations, generating kynurenic acid from kynurenine, thereby antagonizing NMDA receptors (but not likely presynaptic alpha 7 AChRs), and blocking the release of proinflammatory cytokines by microglia via GPCR-35 antagonism. Human CCBL1 is 422 amino acids (aa) in length, and contains one aspartate aminotransferase-like region (aa 31-417). There are three potential splice variants, one that shows a deletion of aa 68-117, a second that shows a deletion of aa 251-422, and a third that contains a 21 aa substitution for aa 230-250. While the 422 aa isoform is considered cytosolic, there is the potential for a 34 aa N-terminal extension that would act as a mitochondrial targeting sequence. Such a sequence is reported for rat and nonhuman primate. Over aa 182-422, human CCBL1 shares 82% aa sequence identity with mouse CCBL1.
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