FABP1/L-FABP Antibody (328605)

R&D Systems | Catalog # MAB29641

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 328605
View all FABP1/L-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148

Specificity

Detects human FABP1/L-FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with rhFABP2, -3, -4, -5, -6, -7, or rmFABP9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FABP1/L-FABP Antibody (328605)

Detection of Human FABP1/L‑FABP antibody by Western Blot.

Detection of Human FABP1/L‑FABP by Western Blot.

Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/ L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human, Mouse, and Rat FABP1/L-FABP antibody by Western Blot.

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L-FABP Monoclonal Antibody (Catalog # MAB29641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human FABP1/L-FABP antibody by Simple WesternTM.

Detection of Human FABP1/L‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/ L‑FABP Monoclonal Antibody (Catalog # MAB29641). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human and Mouse FABP1/L-FABP antibody by Simple WesternTM.

Detection of Human and Mouse FABP1/L‑FABP by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14-16 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29641). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for FABP1/L-FABP Antibody (328605)

Application
Recommended Usage

Simple Western

5-10 µg/mL
Sample: Human liver tissue, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue

Western Blot

0.25 µg/mL
Sample: Human liver tissue, human colon tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue

Reviewed Applications

Read 3 reviews rated 4.3 using MAB29641 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP), Z-protein and squalene-and sterol-carrier protein (SCP) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P07148

Additional FABP1/L-FABP Products

Product Documents for FABP1/L-FABP Antibody (328605)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FABP1/L-FABP Antibody (328605)

For research use only

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Citations for FABP1/L-FABP Antibody (328605)

Customer Reviews for FABP1/L-FABP Antibody (328605) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human/Mouse/Rat FABP1/L-FABP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    FABP1/L-FABP Antibody (328605) MAB29641
  • Human/Mouse/Rat FABP1/L-FABP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    FABP1/L-FABP Antibody (328605) MAB29641
  • Human/Mouse/Rat FABP1/L-FABP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

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