Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antibody

Catalog # Availability Size / Price Qty
AF7879
AF7879-SP
Detection of Human Golgi Glycoprotein 1/GLG1 by Western Blot.
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Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Golgi Glycoprotein 1/GLG1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Golgi Glycoprotein 1/GLG1
Lys1048-Asn1145
Accession # Q92896
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Golgi Glycoprotein 1/GLG1 by Western Blot. View Larger

Detection of Human Golgi Glycoprotein 1/GLG1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7879) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Golgi Glycoprotein 1/GLG1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry Golgi Glycoprotein 1/GLG1 in PC‑12 Rat Cell Line. View Larger

Golgi Glycoprotein 1/GLG1 in PC‑12 Rat Cell Line. Golgi Glycoprotein 1/GLG1 was detected in immersion fixed PC‑12 rat adrenal pheochromocytoma cell line using Sheep Anti-Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7879) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the Golgi complex. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Golgi  Glycoprotein 1/GLG1 in Human Astrocytoma. View Larger

Golgi Glycoprotein 1/GLG1 in Human Astrocytoma. Golgi Glycoprotein 1/GLG1 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Sheep Anti-Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7879) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Golgi apparatus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Golgi Glycoprotein 1/GLG1

GLG1 (Golgi complex-Localized Glycoprotein 1; also CFR1, E-Selectin ligand-1/ESL-1, MG-160 and Cys-rich FGF receptor) is a 150-160 kDa (reducing; 130 kDa nonreducing) glycoprotein. It is expressed in both Golgi and/or the cell membrane of multiple cell types, including neutrophils (from rodents; not humans), liver stellate cells, neurons, cardiac myocytes, monocytes and bronchial epithelial cells. In the blood, GLG1/ESL-1 collaborates with PSGL-1 to mediate leukocyte binding to endothelial cell surfaces. PSGL-1 initiates leukocyte tethering while GLG1 promotes slow rolling. GLG1 also serves as an intra-Golgi receptor for multiple FGFs, including FGF-1, -2, -4, -18 and possibly -3, and as a component of an unusual latent TGF-beta complex. Mature human GLG1 is an 1150 amino acid (aa) type I transmembrane protein. It contains a 1116 aa extracellular/luminal region (aa 30-1145) plus a short 13 aa cytoplasmic segment. The extracellular region possesses a 16 aa poly-Gln segment followed by 16 Cys-rich repeats (aa 116-1101). There are three potential isoform variants, one of which possess a 24 aa extension at the C‑terminus, a second that couples the aforementioned C‑terminal extension to a deletion of aa 147-157, and a third that contains a 14 aa substitution for aa 685-1179. It is suggested that the longer C‑terminus retains GLG1 in the Golgi, while shorter cytoplasmic segments allow for presentation at the cell membrane. Over aa 1048‑1145, human and mouse are identical in aa sequence.

Entrez Gene IDs
2734 (Human); 20340 (Mouse); 29476 (Rat)
Alternate Names
CFR1; CFR-1; cysteine-rich fibroblast growth factor receptor; DKFZp686L08213; E-selectin ligand 1; ESL1; ESL-1; FLJ23319; FLJ23967; FLJ32797; GLG1; Golgi apparatus protein 1; Golgi Glycoprotein 1; golgi sialoglycoprotein MG-160; MG160; MG-160

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Cell and Tissue Staining Kits

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antibody

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Human/Mouse/Rat Golgi Glycoprotein 1/GLG1 Antibody
By Anonymous on 12/10/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Cells that had verified Glg1 overexpression or knockdown/knockout were lysed and probed with this antibody at 1:1000 in combination with Beta-actin. Glg1 was not detectable even at a very high exposure (Image) *R&D Systems Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*