Human/Mouse/Rat GSK-3 alpha / beta Antibody

Catalog # Availability Size / Price Qty
AF2157
AF2157-SP
Human/Mouse/Rat GSK-3 alpha / beta  Antibody in Data
3 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (4)

Human/Mouse/Rat GSK-3 alpha / beta Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GSK-3 beta and the closely related GSK-3 alpha.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GSK-3 beta
Accession # P49841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GSK‑3 alpha / beta  by Western Blot. View Larger

Detection of Human GSK‑3 alpha / beta by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT-29 human colon adenocarcinoma cell line, MDA-MB-468 and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/ Rat GSK-3a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for GSK-3a/ beta at approximately 46 and 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GSK‑3 alpha / beta  in HeLa Human Cell Line. View Larger

GSK‑3 alpha / beta in HeLa Human Cell Line. GSK-3a/ beta was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat GSK-3a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2157) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of GSK‑3 alpha / beta  in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of GSK‑3 alpha / beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human/Mouse/Rat GSK-3a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2157, filled histogram) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 alpha/beta

GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.

Long Name
Glycogen Synthase Kinase 3
Alternate Names
DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK-3 alpha/beta

Product Datasheets

Citations for Human/Mouse/Rat GSK-3 alpha / beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition
    Authors: D Brethour, M Mehrabian, D Williams, X Wang, F Ghodrati, S Ehsani, EA Rubie, JR Woodgett, J Sevalle, Z Xi, E Rogaeva, G Schmitt-Ul
    Sci Rep, 2017;7(0):40313.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. GSK3beta regulates physiological migration of stem/progenitor cells via cytoskeletal rearrangement.
    Authors: Lapid K, Itkin T, D'Uva G, Ovadya Y, Ludin A, Caglio G, Kalinkovich A, Golan K, Porat Z, Zollo M, Lapidot T
    J Clin Invest, 2013;123(4):1705-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat GSK-3 alpha / beta Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human/Mouse/Rat GSK-3 alpha / beta Antibody
By Anonymous on 06/06/2019
Application: WB Sample Tested: Lung single-cell suspension Species: Mouse

Human/Mouse/Rat GSK-3 alpha / beta Antibody
By Anonymous on 09/14/2018
Application: WB Sample Tested: Colon cancer cell line Species: Human

Human/Mouse/Rat GSK-3 alpha / beta Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Human/Mouse/Rat GSK-3 alpha / beta Antibody
By Anonymous on 12/27/2017
Application: WB Sample Tested: Pancreatic cancer cells Species: Human