Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HABP1/C1QBP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HABP1/C1QBP 
Leu72-Gln279
Accession # NP_031599

Specificity

Detects human, mouse, and rat HABP1/C1QBP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human C1QR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat HABP1/C1QBP Antibody

Detection of Human/Mouse/Rat HABP1/C1QBP antibody by Western Blot.

Detection of Human/Mouse/Rat HABP1/C1QBP by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human liver tissue, mouse brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat HABP1/C1QBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5359) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HABP1/C1QBP at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human and Mouse HABP1/C1QBP antibody by Simple WesternTM.

Detection of Human and Mouse HABP1/C1QBP by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for HABP1/C1QBP at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat HABP1/C1QBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5359) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat HABP1/C1QBP Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and mouse brain (cortex) tissue

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, human liver tissue, mouse brain tissue and rat testis tissue

Reviewed Applications

Read 1 review rated 3 using AF5359 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HABP1/C1QBP

HABP1 (hyaluronan binding protein1; also p32 and gC1qR) is a 33 kDa member of the MAM33 family of proteins. It is widely expressed and found on/in endothelial cells, platelets and dendritic cells. It has multiple binding partners, including complement C1q, hyaluronan, vitronectin and ARF. Full-length mouse HABP1 proprecursor is 279 amino acid (aa) in length. It contains a 70 aa mitochondrial targeting sequence (aa 1‑70) that is cleaved to generate a 209 aa mature segment. A hyaluronan binding site lies between Lys116‑Lys125 and a Tyr phosphorylation site exists at Tyr184. Although the prosegment is usually cleaved, the intact proprecursor is associated with sperm. HABP1 reportedly forms noncovalent homotrimers. Over aa 72‑279, mouse HABP1 shares 99% and 91% aa identity with rat and human HABP1, respectively.

Long Name

Hyaluronic Acid-binding Protein

Alternate Names

C1QBP, gC1qBP, gC1qR, SF2p32

Entrez Gene IDs

708 (Human); 12261 (Mouse)

Gene Symbol

C1QBP

UniProt

NP_031599

Additional HABP1/C1QBP Products

Product Documents for Human/Mouse/Rat HABP1/C1QBP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HABP1/C1QBP Antibody

For research use only

Citations for Human/Mouse/Rat HABP1/C1QBP Antibody

Customer Reviews for Human/Mouse/Rat HABP1/C1QBP Antibody (1)

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  • Human/Mouse/Rat HABP1/C1QBP Antibody
    Name: Simon Liu
    Application: Immunohistochemistry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 12/08/2018
    Human/Mouse/Rat HABP1/C1QBP Antibody AF5359

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