Human/Mouse/Rat HABP1/C1QBP Antibody
Human/Mouse/Rat HABP1/C1QBP Antibody Summary
Accession # NP_031599
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat HABP1/C1QBP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human liver tissue, mouse brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat HABP1/C1QBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5359) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HABP1/C1QBP at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human and Mouse HABP1/C1QBP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for HABP1/C1QBP at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat HABP1/C1QBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5359) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HABP1 (hyaluronan binding protein1; also p32 and gC1qR) is a 33 kDa member of the MAM33 family of proteins. It is widely expressed and found on/in endothelial cells, platelets and dendritic cells. It has multiple binding partners, including complement C1q, hyaluronan, vitronectin and ARF. Full-length mouse HABP1 proprecursor is 279 amino acid (aa) in length. It contains a 70 aa mitochondrial targeting sequence (aa 1‑70) that is cleaved to generate a 209 aa mature segment. A hyaluronan binding site lies between Lys116‑Lys125 and a Tyr phosphorylation site exists at Tyr184. Although the prosegment is usually cleaved, the intact proprecursor is associated with sperm. HABP1 reportedly forms noncovalent homotrimers. Over aa 72‑279, mouse HABP1 shares 99% and 91% aa identity with rat and human HABP1, respectively.
Citation for Human/Mouse/Rat HABP1/C1QBP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Proapoptotic peptide-mediated cancer therapy targeted to cell surface p32.
Authors: Agemy, Lilach, Kotamraju, Venkata, Friedmann-Morvinski, Dinorah, Sharma, Shweta, Sugahara, Kazuki N, Ruoslahti, Erkki
Mol Ther, 2013-08-20;21(12):2195-204.
Sample Types: Cell Lysates
Applications: Western Blot
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