Human/Mouse/Rat Hepassocin Antibody

Catalog # Availability Size / Price Qty
AF1614
AF1614-SP
Detection  of Human, Mouse, and Rat Hepassocin by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Rat Hepassocin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Hepassocin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin
Met1-Ile312
Accession # BAB70690
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat Hepassocin by Western Blot. View Larger

Detection of Human, Mouse, and Rat Hepassocin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/
Mouse/Rat Hepassocin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin homodimer at approximately 65 kDa (as indicated). Hepassocin is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Hepassocin in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Hepassocin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity (1).

References
  1. 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
Entrez Gene IDs
2267 (Human); 246186 (Rat)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

Product Datasheets

Citation for Human/Mouse/Rat Hepassocin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot.
    Authors: Rijken DC, Dirkx SP, Luider TM, Leebeek FW
    Biochem. Biophys. Res. Commun., 2006;350(1):191-4.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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