Human/Mouse/Rat Histone H2AY Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Histone H2AY in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 866416
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Histone H2AY
    Gly230-Thr348
    Accession # O75367
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Histone H2AY by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone H2AY at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Histone H2AY in HeLa Human Cell Line. Histone H2AY was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H2AY
Histone H2AY, also known as macro H2A1 (MH2A1) and H2AFY, is a 40 kDa variant of the histone family, basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Ubiquitously expressed, H2AY replaces conventional H2A histones in a subset of nucleosomes, where it represses transcription and participates in stable X chromosome inactivation. H2AY expression is also enriched in senescence-associated heterochromatin. Alternative splicing creates multiple transcript variants, encoding three distinct isoforms. H2AY expressed in blood has been identified as a potential biomarker associated with the activity and therapeutic response of Huntington disease, an autosomal dominant neurodegenerative disorder. Over amino acids 230-348, human H2AY shares 98% identity with mouse and rat H2AY.
  • Long Name:
    H2A Histone Family, Member Y
  • Entrez Gene IDs:
    9555 (Human); 269141 (Mouse)
  • Alternate Names:
    core histone macro-H2A.1; H2A histone family, member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2AFY; Histone H2A.y; Histone H2AY; Histone MacroH2A1; histone macroH2A1.1; histone macroH2A1.2; MACROH2A1; MACROH2A1.1; macroH2A1.2; Medulloblastoma Antigen MU-MB-50.205; mH2A1
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