Human/Mouse/Rat HSPA2 Antibody

Catalog #: MAB6010 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6010
MAB6010-SP

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Detection of Human, Mouse, and Rat HSPA2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSPA2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat HSPA2 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 520608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HSPA2
Lys529-Gly616
Accession # P54652

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat HSPA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSPA2 Monoclonal Antibody (Catalog # MAB6010) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPA2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSPA2

Heat shock protein A2 (HSPA2), also known as HSP70.2, is a 70 kDa testis specific protein. HSPA2 is required for spermatogenesis and functions as a chaperone for the spermatid DNA packaging proteins TP1 and TP2. HSPA2 is also expressed in cancer cells where it is normally localized to the cytoplasm but migrates to the nucleus during heat shock. Within aa 529‑616, human HSP70A2 shares 99% aa sequence identity with mouse and rat HSPA2.

Long Name

Heat Shock Protein A2

Entrez Gene IDs

3306 (Human); 15512 (Mouse); 60460 (Rat)

Alternate Names

Heat shock 70 kDa protein 2; heat shock 70kD protein 2; heat shock 70kDa protein 2; heat shock-related 70 kDa protein 2; HSP70-2; HSP70-3; HSPA2