Heat shock protein A2 (HSPA2), also known as HSP70.2, is a 70 kDa testis specific protein. HSPA2 is required for spermatogenesis and functions as a chaperone for the spermatid DNA packaging proteins TP1 and TP2. HSPA2 is also expressed in cancer cells where it is normally localized to the cytoplasm but migrates to the nucleus during heat shock. Within aa 529‑616, human HSP70A2 shares 99% aa sequence identity with mouse and rat HSPA2.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 520608
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Product Specifications
Immunogen
E. coli-derived recombinant human HSPA2
Lys529-Gly616
Accession # P54652
Lys529-Gly616
Accession # P54652
Specificity
Detects human, mouse, and rat HSPA2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat HSPA2 Antibody
Detection of Human, Mouse, and Rat HSPA2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSPA2 Monoclonal Antibody (Catalog # MAB6010) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPA2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of HSPA2 by Western Blot
HSP70 regulated by spermine protected against testicular toxicity induced by TP in mice and TM4 cells. a mRNA levels of HSP70s after treatment with spermine or eflornithine, a polyamine pathway inhibitor. b The function of HSP70 was indicated by cell viability, MDA, and LDH levels after treated with a HSP70 inhibitor VER155008. The concentration of spermine was 6.25 μM. c Spermine increased protein levels of HSP70s and eflornithine decreased protein levels of HSP70s. d VER155008 reduced the protective effect of spermine in mice. EF: eflornithine. The concentration of TP used in this experiment was 80 nM in TM4 cell. *P < 0.05, **P < 0.01, and ***P < 0.001 (n = 6) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34758869), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse/Rat HSPA2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Reviewed Applications
Read 2 reviews rated 1.5 using MAB6010 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HSPA2
Long Name
Heat Shock Protein A2
Alternate Names
HSP70-2
Gene Symbol
HSPA2
UniProt
Additional HSPA2 Products
Product Documents for Human/Mouse/Rat HSPA2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat HSPA2 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse/Rat HSPA2 Antibody
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Customer Reviews for Human/Mouse/Rat HSPA2 Antibody (2)
1.5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: Cell culture supernatantSpecies: HumanVerified Customer | Posted 10/14/2025We have tried this antibody on the same sample several times, and have frequently seen this weird negative band as well as low staining
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. -
Application: Western BlotSample Tested: Tumor cell lyastesSpecies: HumanVerified Customer | Posted 08/12/2025
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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