Human/Mouse/Rat HSPA2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat HSPA2 by Western Blot.
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Product Details
Supplemental Products

Human/Mouse/Rat HSPA2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat HSPA2 in Western blots.
Monoclonal Mouse IgG1 Clone # 520608
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HSPA2
Accession # P54652
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat HSPA2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat HSPA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSPA2 Monoclonal Antibody (Catalog # MAB6010) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPA2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSPA2

Heat shock protein A2 (HSPA2), also known as HSP70.2, is a 70 kDa testis specific protein. HSPA2 is required for spermatogenesis and functions as a chaperone for the spermatid DNA packaging proteins TP1 and TP2. HSPA2 is also expressed in cancer cells where it is normally localized to the cytoplasm but migrates to the nucleus during heat shock. Within aa 529‑616, human HSP70A2 shares 99% aa sequence identity with mouse and rat HSPA2.

Long Name
Heat Shock Protein A2
Entrez Gene IDs
3306 (Human); 15512 (Mouse); 60460 (Rat)
Alternate Names
Heat shock 70 kDa protein 2; heat shock 70kD protein 2; heat shock 70kDa protein 2; heat shock-related 70 kDa protein 2; HSP70-2; HSP70-3; HSPA2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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