Human/Mouse/Rat IKK epsilon Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat IKK epsilon.
  • Source
    Monoclonal Mouse IgG1 Clone # 346909
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IKK epsilon
    Met1-Val716
    Accession # Q14164
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IKK epsilon by Western Blot. Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IKK epsilon at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
IKK epsilon in MCF-7 Human Cell Line. IKK epsilon was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
IKK epsilon in Human PBMCs. IKK epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human/Mouse/Rat IKK epsilon Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK epsilon

I kappa B kinase epsilon (IKK epsilon ) is also known as IKBKE and IKKi. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK epsilon is an IKK-related kinase with 30% structural homology to IKK alpha and beta, and is present in a subset of IKK complexes. IKK epsilon activation has been implicated in the initiation of anti-viral gene expression and in the pathogenesis of breast cancer.

  • Long Name:
    IkB Kinase epsilon
  • Entrez Gene IDs:
    9641 (Human); 56489 (Mouse)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase epsilon; IKBKE; IKK epsilon; IKK-E; IKKEMGC125297; IKK-epsilon; IKKI; IKK-I; IKK-iMGC125295; Inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; KIAA0151IKK-related kinase epsilon; MGC125294
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