Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Coral - Stylophora pistillata (Hood Coral), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all JNK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human JNK1
Met1-Gln384
Accession # P45983

Specificity

Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat JNK Pan Specific Antibody

Detection of Human/Mouse/Rat JNK antibody by Western Blot.

Detection of Human/Mouse/Rat JNK by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of recombinant JNK family members antibody by Western Blot.

Detection of recombinant JNK family members by Western Blot.

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
JNK antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

JNK in Human Prostate Cancer Tissue.

JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
JNK antibody in Rat Kidney by Immunohistochemistry (IHC-Fr).

JNK in Rat Kidney.

JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human JNK by Simple WesternTM.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human JNK1/2/3 by Western Blot

Detection of Human JNK1/2/3 by Western Blot

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human JNK1/2/3 by Western Blot

Detection of Human JNK1/2/3 by Western Blot

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human JNK1/2/3 by Western Blot

Detection of Human JNK1/2/3 by Western Blot

Detection of JNK activation in human fibroblasts and corals.Immunoblot revealing activated (pJNK) and total forms of JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata nubbins incubated in the absence (control; ct) or presence of SP600125 (SP), anisomycin (Ani), or combination of both (Ani + SP). Molecular weight standards in kilodaltons (kDa) are indicated on the right side of the blots. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat JNK Pan Specific Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat kidney and thymus, and immersion fixed paraffin-embedded sections of human prostate cancer tissue

Simple Western

10 µg/mL
Sample: HEK293T human embryonic kidney cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 2 reviews rated 5 using AF1387 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JNK

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

UniProt

P45983

Additional JNK Products

Product Documents for Human/Mouse/Rat JNK Pan Specific Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat JNK Pan Specific Antibody

For research use only

Citations for Human/Mouse/Rat JNK Pan Specific Antibody

Customer Reviews for Human/Mouse/Rat JNK Pan Specific Antibody (2)

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  • Human/Mouse/Rat JNK Pan Specific Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    Human/Mouse/Rat JNK Pan Specific Antibody AF1387
  • Human/Mouse/Rat JNK Pan Specific Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/17/2018
    Human/Mouse/Rat JNK Pan Specific Antibody AF1387

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Protocols

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