Human/Mouse/Rat JNK2 Antibody

    
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat JNK2 in Western blots. Recognizes p54 JNK (isoforms 1 and/or 4, both 424 aa) and p46 JNK (isoforms 2 and/or 3, both 382 aa) in Western blots. Because the peptide immunogen corresponds to a region of JNK2 with low homology to other JNKs, the antibody does not detect recombinant JNK1 or JNK3.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Human, Mouse, and Rat JNK2 synthetic peptide
    PGIKFEELFPDWIFPSESERD
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse JNK2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). specific bands for JNK2 was detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of JNK2 by Western Blot. Western blot shows recombinant JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for JNK2 was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JNK2
The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.
  • Long Name:
    C-Jun N-terminal Kinase 2
  • Entrez Gene IDs:
    5601 (Human); 243911 (Mouse); 50658 (Rat)
  • Alternate Names:
    c-Jun kinase 2; c-Jun N-terminal kinase 2; EC 2.7.11; EC 2.7.11.24; JNK2; JNK2A; JNK2BETA; JNK2JNK2ALPHA; JNK-55; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; mitogen-activated protein kinase 9; p54a; p54aSAPK; PRKM9JNK2B; SAPK; SAPK1 alpha; Stress-activated protein kinase JNK2
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 46  
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Normal Rabbit IgG Control

Ctrl , CyTOF-ready MAB1050  
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 35  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL004 10
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002 1  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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