Detects human, mouse, and rat JNK2 in Western blots. Recognizes p54 JNK (isoforms 1 and/or 4, both 424 aa) and p46 JNK (isoforms 2 and/or 3, both 382 aa) in Western blots. Because the peptide immunogen corresponds to a region of JNK2 with low homology to other JNKs, the antibody does not detect recombinant JNK1 or JNK3.
Polyclonal Rabbit IgG
Human, Mouse, and Rat JNK2 synthetic peptide PGIKFEELFPDWIFPSESERD
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse JNK2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). specific bands for JNK2 was detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of JNK2 by Western Blot. Western blot shows recombinant JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1846) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for JNK2 was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.