Detection of Human MIOS by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MIOS is a member of the
octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs)
complex. MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L
and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3. This
inhibition by GATOR2 positively regulates mTORC1 signaling, an activity
modulated by energy levels, growth factors, and amino acids. Human MIOS
is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and
a C-terminal zinc finger-like domain. Over aa 620-875, human MIOS shares
98% identity with mouse and rat MIOS.
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