MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex. MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3. This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids. Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain. Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.
MIOS Antibody (955413)
R&D Systems | Catalog # MAB9289
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 955413
Loading...
Product Specifications
Immunogen
Human MIOS synthetic peptide
Accession # Q9NXC5
Accession # Q9NXC5
Specificity
Detects human MIOS in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MIOS Antibody (955413)
Detection of Human MIOS by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for MIOS Antibody (955413)
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MIOS
Long Name
Missing Oocyte, Meiosis Regulator
Alternate Names
MIO
Gene Symbol
MIOS
UniProt
Additional MIOS Products
Product Documents for MIOS Antibody (955413)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for MIOS Antibody (955413)
For research use only
Citations for MIOS Antibody (955413)
Powered by Bioz
Customer Reviews for MIOS Antibody (955413)
There are currently no reviews for this product. Be the first to review MIOS Antibody (955413) and earn rewards!
Have you used MIOS Antibody (955413)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...