Human/Mouse/Rat MIOS Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human MIOS in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 955413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human MIOS synthetic peptide
    Accession # Q9NXC5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MIOS by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIOS

MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex.  MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3.  This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids.  Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain.  Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.

  • Long Name:
    Missing Oocyte, Meiosis Regulator
  • Entrez Gene IDs:
    54468 (Human); 252875 (Mouse); 362324 (Rat)
  • Alternate Names:
    FLJ20323; MIO; MIOS; missing oocyte, meiosis regulator, homolog (Drosophila); WD repeat-containing protein mio
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 99  
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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