Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Yeast

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527

Specificity

Detects human, mouse and rat p27.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat p27/Kip1 Antibody

Detection of Human p27/Kip1 antibody by Western Blot.

Detection of Human p27/Kip1 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of p27/Kip1in Human PBMCs antibody by Flow Cytometry.

Detection of p27/Kip1in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were stained with Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human/Mouse/Rat p27/Kip1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line

Reviewed Applications

Read 1 review rated 4 using AF2256 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Gene Symbol

CDKN1B

UniProt

P46527

Additional p27/Kip1 Products

Product Documents for Human/Mouse/Rat p27/Kip1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p27/Kip1 Antibody

For research use only

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Citations for Human/Mouse/Rat p27/Kip1 Antibody

Customer Reviews for Human/Mouse/Rat p27/Kip1 Antibody (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Human/Mouse/Rat p27/Kip1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat p27/Kip1 Antibody AF2256

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Associated Pathways

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