Human/Mouse/Rat Peroxiredoxin 2 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    The antibody detects endogenous human, mouse and rat Peroxiredoxin 2 in in Western blots. In Western blots, approximately
    25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Peroxiredoxin 2
    Met1-Asn198
    Accession # P32119
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human Peroxiredoxin 2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 2 at approximately 27 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 2
Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.
  • Entrez Gene IDs:
    7001 (Human); 21672 (Mouse); 29338 (Rat)
  • Alternate Names:
    EC 1.11.1; MGC4104; Natural killer cell-enhancing factor B; natural killer-enhancing factor B; NKEF-B; NKEFBNKEF-B; Peroxiredoxin 2; peroxiredoxin-2; PRDX2; PRPTDPX1; PRX2; PRXII; TDPX1; thiol-specific antioxidant 1; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; torin; TPX1; TSA; TSAEC 1.11.1.15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling.
    Authors: Sobotta M, Liou W, Stocker S, Talwar D, Oehler M, Ruppert T, Scharf A, Dick T
    Nat Chem Biol, 2015;11(1):64-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat Peroxiredoxin 2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information