Human/Mouse/Rat Peroxiredoxin 2 Antibody

Catalog # Availability Size / Price Qty
AF3489
AF3489-SP
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot.
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Product Details
Citations (4)
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Human/Mouse/Rat Peroxiredoxin 2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous human, mouse and rat Peroxiredoxin 2 in in Western blots. In Western blots, approximately
25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Peroxiredoxin 2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western Detection of Human Peroxiredoxin 2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Peroxiredoxin 2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Peroxiredoxin 2 at approximately 27 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 2

Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.

Entrez Gene IDs
7001 (Human); 21672 (Mouse); 29338 (Rat)
Alternate Names
EC 1.11.1; MGC4104; Natural killer cell-enhancing factor B; natural killer-enhancing factor B; NKEF-B; NKEFBNKEF-B; Peroxiredoxin 2; peroxiredoxin-2; PRDX2; PRPTDPX1; PRX2; PRXII; TDPX1; thiol-specific antioxidant 1; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; torin; TPX1; TSA; TSAEC 1.11.1.15

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Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Miro1-mediated mitochondrial positioning supports subcellular redox status
    Authors: H Alshaabi, N Shannon, R Gravelle, S Milczarek, T Messier, B Cunniff
    Redox Biology, 2021;38(0):101818.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells
    Authors: KT Stein, SJ Moon, AN Nguyen, HD Sikes
    PLoS Comput. Biol., 2020;16(9):e1008202.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. A role for peroxiredoxins in H2O2- and MEKK-dependent activation of the p38 signaling pathway
    Authors: AG Barata, TP Dick
    Redox Biol, 2019;28(0):101340.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling.
    Authors: Sobotta M, Liou W, Stocker S, Talwar D, Oehler M, Ruppert T, Scharf A, Dick T
    Nat Chem Biol, 2015;11(1):64-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

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Secondary Antibodies

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