Human/Mouse/Rat Phospho-ATM (S1981) Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human ATM when phosphorylated at S1981. Also detects the comparable phosphorylated sites in mouse ATM (S1987) and rat ATM (S1952).
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human ATM S1981 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda ‑phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-ATM (S1981) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATM

The Ataxia telangiectasia-mutated (ATM) protein kinase exists as a dimer in the cell nucleus. Changes in DNA structure induced by genotoxic stress lead to activation of ATM and phosphorylation of S1981 in trans. Once S1981 is phosphorylated, the dimer dissociates and active ATM monomers signal to downstream targets.

  • Long Name:
    Ataxia Telangiectasia-mutated
  • Entrez Gene IDs:
    472 (Human); 11920 (Mouse)
  • Alternate Names:
    AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. DEK is required for homologous recombination repair of DNA breaks
    Authors: EA Smith, B Gole, NA Willis, R Soria, LM Starnes, EF Krumpelbec, AG Jegga, AM Ali, H Guo, AR Meetei, PR Andreassen, F Kappes, LM Vinnedge, JA Daniel, R Scully, L Wiesmüller, SI Wells
    Sci Rep, 2017;7(0):44662.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival.
    Authors: Cornell L, Munck J, Alsinet C, Villanueva A, Ogle L, Willoughby C, Televantou D, Thomas H, Jackson J, Burt A, Newell D, Rose J, Manas D, Shapiro G, Curtin N, Reeves H
    Clin Cancer Res, 2015;21(4):925-33.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  3. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
    Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
    Nat Commun, 2014;2(0):4172.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Direct activation of ATM by resveratrol under oxidizing conditions.
    Authors: Lee, Ji-Hoon, Guo, Zhi, Myler, Logan R, Zheng, Suting, Paull, Tanya T
    PLoS ONE, 2014;9(6):e97969.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia.
    Authors: Byrd PJ, Srinivasan V, Last JI
    Br. J. Cancer, 2012;106(2):262-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours.
    Authors: Reiman A, Srinivasan V, Barone G
    Br. J. Cancer, 2011;105(4):586-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.
    Authors: Rajesh P, Rajesh C, Wyatt MD
    DNA Repair (Amst.), 2010;9(4):458-67.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells.
    Authors: Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT
    Biochem. Pharmacol., 2009;77(5):770-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
    Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
    J. Cell Biol., 2008;183(3):385-91.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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