Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody

Name: Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody
Brand: R&D Systems
SKU: MAB74541
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB74541

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1016A

View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human/mouse/rat PLC-gamma 1 Y783 site
Accession # P19174

Specificity

Detects human/mouse/rat PLC-gamma 1 when phosphorylated at Y783 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody

Detection of Human Phospho-PLC‑ gamma 1 (Y783) by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB74541) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody

Application

Recommended Usage

Western Blot

0.05 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PLC-gamma 1

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones, and phosphorylation at Tyr783 is a widely used indicator of activation. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Entrez Gene IDs

5335 (Human); 18803 (Mouse); 25738 (Rat)

Gene Symbol

PLCG1

UniProt

P19174

Additional PLC-gamma 1 Products

Product Documents for Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Phospho-PLC‑ gamma 1 (Y783) Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only