Human/Mouse/Rat Phospho-PTEN (S380) Antibody

Catalog # Availability Size / Price Qty
AF2838
AF2838-SP
Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot.
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Citations (2)
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Human/Mouse/Rat Phospho-PTEN (S380) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PTEN when phosphorylated at S380 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human PTEN S380 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot. View Larger

Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot.

Western blot shows Human/Mouse/Rat PTEN Monoclonal Antibody (Catalog # MAB847) immunopreciptate of MRC‑5 human embryonic lung fibroblast cell line, mouse and rat brain tissue untreated (-) or treated (+) with 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/
Rat Phospho-PTEN (S380) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2838), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PTEN (S380) at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTEN

The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

References
  1. Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
  2. Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
  3. Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
  4. Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.
Long Name
Phosphatase and Tensin Homolog Deleted on Chromosome 10
Entrez Gene IDs
5728 (Human); 19211 (Mouse); 50557 (Rat)
Alternate Names
BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM

Product Datasheets

Citations for Human/Mouse/Rat Phospho-PTEN (S380) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Blockade of Axl signaling ameliorates HPV16E6-mediated tumorigenecity of cervical cancer
    Authors: EH Lee, KY Ji, EM Kim, SM Kim, HW Song, HR Choi, BY Chung, HJ Choi, HW Bai, HS Kang
    Sci Rep, 2017;7(1):5759.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expression.
    Authors: Kechagioglou, Petros, Papi, Rigini M, Provatopoulou, Xeni, Kalogera, Eleni, Papadimitriou, Elli, Grigoropoulos, Petros, Nonni, Aphrodit, Zografos, George, Kyriakidis, Dimitrio, Gounaris, Antonia
    Anticancer Res, 2014;34(3):1387-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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