Human/Mouse/Rat PLC-gamma 1 Antibody

Catalog # Availability Size / Price Qty
AF3288
AF3288-SP
Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.
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Human/Mouse/Rat PLC-gamma 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PLC-gamma 1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLC-gamma 1
Ser1091-Leu1290
Accession # P19174
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot. View Larger

Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC-gamma 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLC-gamma 1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-gamma 1

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.

Long Name
Phospholipase C gamma 1
Entrez Gene IDs
5335 (Human); 18803 (Mouse); 25738 (Rat)
Alternate Names
EC 3.1.4.11; gamma 1 (formerly subtype 148); monophosphatidylinositol phosphodiesterase; NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; PLC148; PLC-148; PLCG1; PLCgamma 1; PLC-gamma 1; PLC-gamma-1; PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; PPLCA; triphosphoinositide phosphodiesterase

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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