Human/Mouse/Rat PON3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat PON3 by Western Blot.
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Product Details
Citations (2)
Supplemental Products
Reviews (4)

Human/Mouse/Rat PON3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody did not cross‑react with recombinant human PON1 or PON2.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PON3
Accession # Q15166
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PON3 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PON3 by Western Blot. Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PON3

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.

Long Name
Paraoxonase 3
Entrez Gene IDs
5446 (Human); 269823 (Mouse); 312086 (Rat)
Alternate Names
EC; EC; EC; paraoxanase-3; paraoxonase 3; PON3; serum paraoxonase/lactonase 3

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Citations for Human/Mouse/Rat PON3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death.
    Cell Death Differ., 2012;19(9):1549-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Paraoxonase 2 is down-regulated by the Pseudomonas aeruginosa quorumsensing signal N-(3-oxododecanoyl)-L-homoserine lactone and attenuates oxidative stress induced by pyocyanin.
    Authors: Horke S, Witte I, Altenhofer S, Wilgenbus P, Goldeck M, Forstermann U, Xiao J, Kramer GL, Haines DC, Chowdhary PK, Haley RW, Teiber JF
    Biochem. J., 2010;426(1):73-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat PON3 Antibody

Average Rating: 4 (Based on 4 Reviews)

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Human/Mouse/Rat PON3 Antibody
By Anonymous on 02/01/2022
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Mouse

Detect both the endogenous and over-expressed mouse PON3.

Human/Mouse/Rat PON3 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat PON3 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat PON3 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human