The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human PON3
Ala30-Leu354
Accession # Q15166
Ala30-Leu354
Accession # Q15166
Specificity
Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody did not cross‑react with recombinant human PON1 or PON2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PON3 Antibody
Detection of Human/Mouse/Rat PON3 by Western Blot.
Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat PON3 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: human serum, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue,
Sample: human serum, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue,
Reviewed Applications
Read 4 reviews rated 4 using AF4345 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PON3
Long Name
Paraoxonase 3
Alternate Names
EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3
Gene Symbol
PON3
UniProt
Additional PON3 Products
Product Documents for Human/Mouse/Rat PON3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat PON3 Antibody
For research use only
Citations for Human/Mouse/Rat PON3 Antibody
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Customer Reviews for Human/Mouse/Rat PON3 Antibody (4)
4 out of 5
4 Customer Ratings
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Application: Western BlotSample Tested: HEK293 human embryonic kidney cell lineSpecies: MouseVerified Customer | Posted 02/01/2022Detect both the endogenous and over-expressed mouse PON3.
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/07/2018
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/02/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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