Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PON3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PON3
Ala30-Leu354
Accession # Q15166

Specificity

Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody did not cross‑react with recombinant human PON1 or PON2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PON3 Antibody

Detection of Human/Mouse/Rat PON3 antibody by Western Blot.

Detection of Human/Mouse/Rat PON3 by Western Blot.

Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat PON3 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: human serum, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue,

Reviewed Applications

Read 4 reviews rated 4 using AF4345 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PON3

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.

Long Name

Paraoxonase 3

Alternate Names

EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3

Entrez Gene IDs

5446 (Human); 269823 (Mouse); 312086 (Rat)

Gene Symbol

PON3

UniProt

Q15166

Additional PON3 Products

Product Documents for Human/Mouse/Rat PON3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PON3 Antibody

For research use only

Citations for Human/Mouse/Rat PON3 Antibody

Customer Reviews for Human/Mouse/Rat PON3 Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat PON3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Mouse
    Verified Customer | Posted 02/01/2022
    Detect both the endogenous and over-expressed mouse PON3.
    Human/Mouse/Rat PON3 Antibody AF4345
  • Human/Mouse/Rat PON3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Mouse/Rat PON3 Antibody AF4345
  • Human/Mouse/Rat PON3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse/Rat PON3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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