Human/Mouse/Rat RAGE Antibody

Catalog #: AF1179
13 Citations Datasheet / COA / SDS

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AF1179
AF1179-SP

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Detection of Human RAGE by Western Blot.

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Citations (13)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RAGE Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat RAGE in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant canine RAGE is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RAGE
Gln24-Ala342
Accession # O35444

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1-1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 15-35 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE-BSA to immobilized Recombinant Mouse RAGE Fc Chimera (Catalog # 1179-RG) coated at 5 µg/mL (100 µL/well). At 166 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Mouse and Rat RAGE by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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RAGE in Mouse Brain. RAGE was detected in immersion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

References

Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
Yonekura, H. et al. (2003) Biochem. J. 370:1097.
Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
Valencia, J.V. et al. (2004) Diabetes 53:743.

Long Name

Receptor for Advanced Glycation End Products

Entrez Gene IDs

177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)

Alternate Names

advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat RAGE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism
Authors: C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente
Nature Medicine, 2022;28(4):752-765.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Heparan sulfate-dependent RAGE oligomerization is indispensable for pathophysiological functions of RAGE
Authors: M Li, CY Ong, CJ Langouët-A, L Tan, A Verma, Y Yang, X Zhang, DK Shah, EP Schmidt, D Xu
Elife, 2022;11(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages
Authors: A Kitaura, T Nishinaka, S Hamasaki, OF Hatipoglu, H Wake, M Nishibori, S Mori, S Nakao, H Takahashi
PLoS ONE, 2021;16(1):e0245957.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
RAGE is a Critical Mediator of Pulmonary Oxidative Stress, Alveolar Macrophage Activation and Emphysema in Response to Cigarette Smoke
Authors: KA Sanders, DA Delker, T Hueckstead, E Beck, T Wuren, Y Chen, Y Zhang, MW Hazel, JR Hoidal
Sci Rep, 2019;9(1):231.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke
Authors: L Wolf, C Herr, J Niederstra, C Beisswenge, R Bals
PLoS ONE, 2017;12(7):e0180092.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice.
Authors: Goury A, Meghraoui-Kheddar A, Belmokhtar K, Vuiblet V, Ortillon J, Jaisson S, Devy J, Le Naour R, Tabary T, Cohen J, Schmidt A, Rieu P, Toure F
J Immunol, 2015;194(8):3612-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
Authors: Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
Immunity, 2013;38(3):541-54.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism.
Authors: Mallidis C, Czerwiec A, Filippi S, O'Neill J, Maggi M, McClure N
Reproduction, 2011;142(1):63-71.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
Type I epithelial cells are the main target of whole-body hypoxic preconditioning in the lung.
Authors: Zhang SX, Miller JJ, Stolz DB, Serpero LD, Zhao W, Gozal D, Wang Y
Am. J. Respir. Cell Mol. Biol., 2009;40(3):332-9.
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
Authors: Sakatani S, Seto-Ohshima A, Shinohara Y, Yamamoto Y, Yamamoto H, Itohara S, Hirase H
J. Neurosci., 2008;28(43):10928-36.
Species: Rat
Sample Types: In Vivo
Applications: Electrophysiology

Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
J. Clin. Invest., 2008;118(1):183-94.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
Authors: Mitola S, Belleri M, Urbinati C, Coltrini D, Sparatore B, Pedrazzi M, Melloni E, Presta M
J. Immunol., 2006;176(1):12-5.
Species: Chicken
Sample Types: In Ovo
Applications: Neutralization