|Detection of Human/Mouse/Rat Ras-GAP by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Ras‑GAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ras‑GAP at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Ras-GAP (Ras GTPase-activating protein) interacts with Ras to stimulate the otherwise weak intrinsic GTPase activity of Ras and to promote the return of Ras to an inactive GDP-bound state. Thus, Ras-GAP is a negative regulator of Ras. Human Ras-GAP is 1047 amino acids in length. The N-terminal region contains two SH2 domains and an intervening SH3 domain, while the C-terminal half of Ras-GAP is sufficient to accelerate Ras GTPase activity.
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