Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RSK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RSK1
Accession # Q15418

Specificity

Detects human, rat and mouse RSK1. This antibody does not detect recombinant human RSK2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat RSK1 Antibody

Detection of Human/Mouse/Rat RSK1 antibody by Western Blot.

Detection of Human/Mouse/Rat RSK1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF992) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RSK1 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

RSK1 in Human Ovarian Cancer Tissue.

RSK1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human/Mouse/Rat RSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF992) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat RSK1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 4 using AF992 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RSK1

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Entrez Gene IDs

6195 (Human); 20111 (Mouse); 81771 (Rat)

Gene Symbol

RPS6KA1

UniProt

Q15418

Additional RSK1 Products

Product Documents for Human/Mouse/Rat RSK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat RSK1 Antibody

For research use only

Citations for Human/Mouse/Rat RSK1 Antibody

Customer Reviews for Human/Mouse/Rat RSK1 Antibody (1)

4 out of 5
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  • Human/Mouse/Rat RSK1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: hFOB 1.19
    Species: Human
    Verified Customer | Posted 11/30/2022
    Human fetal osteoblasts 1.19 (hFOB 1.19) were lysed with NP-40 buffer for protein extraction. 10 ug of proteins were loaded in a 10% Acrylamide gel.
    Human/Mouse/Rat RSK1 Antibody AF992

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Protocols

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