Human/Mouse/Rat SH2B1 Antibody

Detection of Human, Mouse, and Rat SH2B1 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SH2B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6915) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SH2B1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SH2B1 by Western Blot

Intelligence-related behaviors in mice are dependent on neuronal Sh2b1 expression in the hippocampus. (A) Experimental timeline. (B) Schematics of AAV injections and representative images of GFP expression in injected areas. Scale bar, 200 μm. (C and D) Representative immunoblots (C) and pooled data (D). Sh2b1+/+, n = 5; Sh2b1 delta Ν, n = 6. P = 0.0149 (*), unpaired Student’s t test. (E) Diagram of the Y-maze test. (F to H) Error entries (F), total entries (G), and error rate (%, H). Sh2b1+/+, n = 20; Sh2b1 delta Ν, n = 32. P = 0.0037 (**), 0.2535, and 0.015 (**), for (F), (G), and (H), Sh2b1+/+ versus Sh2b1 delta Ν, unpaired Student’s t test. (I) Diagrams for novel object recognition test. (J to L) Novel object recognition test. Sh2b1+/+, n = 20; Sh2b1 delta Ν, n = 32. (J) Left: Sh2b1+/+, P = 1.0E−4 (***); Right: Sh2b1 delta Ν, P = 0.3014, Familiar versus New, paired Student’s t test. (K and L) P = 0.1114 and 0.0128 (*), for (K) and (L), Sh2b1+/+ versus Sh2b1 delta Ν, unpaired Student’s t test. (M and N) Morris water maze test. (M) Percentage of time spent in the 4 quadrants during the probe trials after learning. (N) Reverse learning curve. Sh2b1+/+, n = 18; Sh2b1 delta Ν, n = 19. Two-way repeated measures ANOVA, main effect of group, F1,35 = 2.577, P = 0.1174. P = 0.0246 (*), 0.3508, 0.2751, and 0.3114, from the first to the fourth day since reverse learning, Sh2b1+/+ versus Sh2b1 delta Ν, unpaired Student’s t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38434247), licensed under a CC-BY license. Not internally tested by R&D Systems.