Human/Mouse/Rat SH2B1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat SH2B1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human/Mouse/Rat SH2B1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat SH2B1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human SH2B1
Accession # Q9NRF2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat SH2B1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat SH2B1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SH2B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6915) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SH2B1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2B1

SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1 alpha, PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80 kDa, it runs anomalously at 90-102 kDa in SDS-Page. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1( alpha ) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa 24-85), an NLS (aa 224-233), one pleckstrin homology region (aa 249-378) and an SH2 domain (aa 521-625). At least three utilized phosphorylation sites exist. There are at least two splice variants for SH2B1. The 90-92 kDa SH2B1 beta isoform shows a 39 aa substitution for aa 633-756, while the SH2B1 gamma isoform possesses a 50 aa substitution for the same aa range (i.e., aa 633-756). Over aa 317-467, human SH2B1 shares 92% aa identity with mouse SH2B1.

Long Name
SH2B adaptor protein 1
Entrez Gene IDs
25970 (Human); 20399 (Mouse)
Alternate Names
FLJ30542; KIAA1299; Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2B adapter protein 1; SH2B adaptor protein 1; SH2-B signaling protein; SH2B1; SH2BDKFZp547G1110

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat SH2B1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat SH2B1 Antibody and earn rewards!

Have you used Human/Mouse/Rat SH2B1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review