SHIP2 (SH2 domain-containing inositol 5-phosphatase 2; also inositol polyphosphate phosphatase-like 1) is a member of the phosphatidylinositol/PtdIns 5-phosphatase family of enzymes. It is widely expressed and negatively regulates PI3 kinase pathways by hydrolyzing the 5-phosphate of PtdIns-3,4,5-triphosphate. Human SHIP2 is 1258 amino acids (aa) in length. It contains one SH2 domain (aa 21‑117), a Pro-rich region with an SH3 binding site (aa 935-1105), and a SAM (or sterile alpha -motif) domain that binds ARAP3 (aa 1196‑1258). SHIP2 is phosphorylated in response to growth factor activation on Tyr986, 1162 and 1358, and on Thr958, among other sites. Over aa 1106‑1258, human SHIP2 shares 95% aa identity with mouse SHIP2.
Human/Mouse/Rat SHIP2 Antibody
R&D Systems | Catalog # AF5389
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human SHIP2
Ala1106-Lys1258
Accession # O15357
Ala1106-Lys1258
Accession # O15357
Specificity
Detects human, mouse and rat SHIP2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat SHIP2 Antibody
Detection of Human/Mouse/Rat SHIP2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, HUVEC human umbilical vein endothelial cells, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SHIP2 at approximately 140 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
SHIP2 in PC‑3 Human Cell Line.
SHIP2 was detected in immersion fixed PC-3 human prostate cancer cell line using Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not yet been tested in mouse or rat samples.
SHIP2 in Human Breast Cancer Tissue.
SHIP2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse/Rat SHIP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5389) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse SHIP2 by Western Blot
SHIP1/2 and PTEN expression is not affected in Ship2 delta / delta mice. Neutrophils from wild-type (WT) and Ship2 delta / delta ( delta / delta ) mice were tested for SHIP1, SHIP2, PTEN, PKB and loading control (HSP90, beta -actin) expression. (A) Representative examples and (B–E) densitometry of 4 (PTEN, PKB, HSP90) or 5 (SHIP1/2, beta -actin) separate experiments performed. Mean ± SEM are presented; AU, arbitrary units. n.s., not significant. (F) A comparison of blood cell counts between wild-type and Ship2 delta / delta mice. RBC, red blood cells; WBC, white blood cells; myelo, myeloid cells; neutro, neutrophils. Every symbol represents data obtained from one mouse. p values were determined by unpaired two-tailed t tests; differences did not reach significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33953730), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse/Rat SHIP2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, HUVEC human umbilical vein endothelial cells, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, HUVEC human umbilical vein endothelial cells, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SHIP2
Long Name
SH2-containing Inositol-5'-Phosphatase 2
Alternate Names
INPPL1
Gene Symbol
INPPL1
UniProt
Additional SHIP2 Products
Product Documents for Human/Mouse/Rat SHIP2 Antibody
Product Specific Notices for Human/Mouse/Rat SHIP2 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse/Rat SHIP2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars