|Detection of Human/Mouse/Rat Thioredoxin‑2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, CH-1 mouse B cell lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Thioredoxin‑2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thioredoxin-2 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.|
Thioredoxin-2 (Trx2), also known as mitochondrial Thioredoxin, belongs to the evolutionarily conserved Thioredoxin family. These proteins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature mouse Thioredoxin‑2 is identical to mature rat Thioredoxin-2 and 98% identical to human Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant‑induced apoptosis.
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