Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody

Catalog # Availability Size / Price Qty
MAB7428
MAB7428-SP
Detection of Thioredoxin Reductase 1/TRXR1 in HeLa Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse Thioredoxin Reductase 1/TRXR1 in direct ELISAs and human, mouse, and rat Thioredoxin Reductase 1/TRXR1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 489804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Thioredoxin Reductase 1/TRXR1
Met1-Ile497
Accession # Q16881
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of Thioredoxin Reductase 1/TRXR1 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Thioredoxin Reductase 1/TRXR1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Western Blot Detection of Human, Mouse, and Rat Thioredoxin Reductase 1/TRXR1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Thioredoxin Reductase 1/TRXR1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin Reductase 1/TRXR1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin Reductase 1/TRXR1

Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family.  Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions.  Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver.

Entrez Gene IDs
7296 (Human); 50493 (Mouse); 58819 (Rat)
Alternate Names
EC 1.8.1; EC 1.8.1.9; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM12; GRIM-12; GRIM-12TR1; KDRF; KM-102-derived reductase-like factor; MGC9145; oxidoreductase; Thioredoxin Reductase 1; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; TR1; TRXR1; TRXRD1; TXNR; TXNRD1; TXNRTRXR1

Product Datasheets

Citation for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor
    Authors: Y Fan, M Makar, MX Wang, HW Ai
    Nat. Chem. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody

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Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody
By Anonymous on 01/10/2018
Application: WB Sample Tested: PC-3 human prostate cancer cell line Species: Human