Human/Mouse/Rat Tmp21/p23 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Tmp21/p23 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 805125
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human Tmp21/p23
    Ile32-Arg185
    Accession # P49755
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Tmp21/p23 by Western Blot. Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, Capan‑1 human pancreatic adenocarcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Tmp21/p23 Monoclonal Antibody (Catalog # MAB7630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tmp21/p23 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tmp21/p23

Tmp21 (Transmembrane protein 21 also TMED-10,Transmembrane emp24 Domain-containing Protein 10, p24δ1 and p23) is a 21‑23 kDa member of the EMP [endosome membrane protein] 24 /GP25L family of cargo molecules. It is ubiquitously expressed, being found associated with the ER-Golgi complex. Molecules destined for secretion are folded/processed in the ER, transported out of the ER via COP-II coated vesicles, united with other molecules in similar vesicles to form an ERGIC/ER-Golgi Intermediate Compartment, and finally delivered to the Golgi (or returned to the ER) via new (presumably COP-I) coated vesicles for terminal maturation. Tmp21 is considered part of both the COP-II and COP-I oligomer complex, the latter that arises from the ERGIC and both stabilizes the ER and allows for the selection of specific molecules targeted for secretion. It also is known to associate with the presenilin complex in the plasma membrane and downregulate gamma ‑secretase activity. This impacts A beta production but not AICD generation. Mature human Tmp21 is a 188 amino acid (aa) type I transmembrane protein. It contains a 154 aa luminal region (aa 32-185) that shows a membrane interaction GOLD (Golgi Dynamics) domain (aa 41-193), and a short 13 aa cytoplasmic tail (aa 207-219) that binds to COP vesicle coat complexes. Exists as both a monomer and homodimer. Over aa 32-185, human Tmp21 shares 97% aa sequence identity with mouse Tmp21.

  • Long Name:
    21 kDa Transmembrane Trafficking Protein
  • Entrez Gene IDs:
    10972 (Human); 68581 (Mouse); 84599 (Rat)
  • Alternate Names:
    21 kDa transmembrane-trafficking protein; p23; P24(DELTA); p24delta; S31I125; S31III125; TMED10; Tmp21; TMP21Tmp-21-I; transmembrane emp24 domain-containing protein 10; transmembrane emp24-like trafficking protein 10 (yeast); Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 31  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 25  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse IgG HRP-conjugated Antibody

WB HAF018 5  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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