Detection of Human, Mouse, and Rat XPB by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, NRK rat normal kidney cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat XPB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPB at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89 kDa subunit) is an 86 kDa, nuclear member of the RAD25/XPB subfamily, helicase family of proteins. It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes. XPB is part of the 10 subunit TFIIH complex. As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters. During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair. Human XPB is 782 amino acids (aa) in length. It contains an NLS (aa 6‑18), multiple acidic-rich regions, an ATPase region (aa 327‑488) and one helicase domain (aa 542‑702). Over aa 1‑375, human XPB shares 93% aa identity with mouse XPB.
Xeroderma Pigmentosum B
Entrez Gene IDs:
2071 (Human); 13872 (Mouse); 291703 (Rat)
Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; EC 3.6.1; EC 126.96.36.199; ERCC3; excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing); GTF2H; RAD25; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC; XPBTFIIH 89 kDa subunit
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