Human/Mouse SDPR Antibody
R&D Systems | Catalog # AF5759
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Gly2-Val180
Accession # O95810
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse SDPR Antibody
Detection of Human and Mouse SDPR by Western Blot.
Western blot shows lysates of human heart and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SDPR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SDPR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of SDPR by Western Blot
Impairment of caveolar invagination by syndapin III KO does not lead to dissociation of CAVIN-1 from the plasma membrane. (c-f) Quantitative western blot analyses of homogenates of hearts (c,d) from WT and syndapin III KO mice addressing components suggested to play roles in caveolae formation that may be redundant or related to the critical role of syndapin III in caveolar invagination (normalized to GAPDH). Data, mean ± SEM. N = 12 each genotype. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29202928), licensed under a CC-BY license. Not internally tested by R&D Systems.Detection of SDPR by Western Blot
Impairment of caveolar invagination by syndapin III KO does not lead to dissociation of CAVIN-1 from the plasma membrane. (e,f) from WT and syndapin III KO mice addressing components suggested to play roles in caveolae formation that may be redundant or related to the critical role of syndapin III in caveolar invagination (normalized to GAPDH). Data, mean ± SEM. N = 12 each genotype. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29202928), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse SDPR Antibody
Western Blot
Sample: Human heart and mouse lung tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SDPR
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SDPR Products
Product Documents for Human/Mouse SDPR Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse SDPR Antibody
For research use only
Related Research Areas
Citations for Human/Mouse SDPR Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars