Human/Mouse SIN3A Antibody

Catalog # Availability Size / Price Qty
AF6115
AF6115-SP
Human/Mouse SIN3A Antibody in Data
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Human/Mouse SIN3A Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SIN3A in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SIN3A
Tyr1187-Pro1273
Accession # Q96ST3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SIN3A antibody by Western Blot. View Larger

Detection of Human SIN3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SIN3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6115) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SIN3A at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIN3A

SIN3A (Switch insensitive-3 A) is a nuclear, 140‑150 kDa member of the SIN3 family of co-repressors. SIN3A plays a key role in gene repression. Normally, genes are not expressed when chromatin is "closed", or condensed, precluding transcriptional activators from assembling on gene promoters. This state is achieved through histone deacetylation and DNA methylation, and SIN3A serves as a scaffold for multiple proteins, including deacetylases (HDAC1 and 2). Human SIN3A is 1273 amino acids (aa) in length. It contains three PAH (paired amphipathic helix) repeats (aa 141‑187, 322‑381 and 477‑523) and multiple phosphoserines and acetylated lysines. There are three potential splice variants. The first shows a 33 aa substtution for aa 124‑1273, while the second and third show eight aa substitutions for aa 337‑352, coupled to either a deletion of aa 1009‑1066, or a two aa substitution for aa 1008‑1065. Over aa 1187‑1273, human and mouse SIN3A show 100% identity in aa sequence.

Long Name
SIN3 Homolog A
Entrez Gene IDs
25942 (Human); 20466 (Mouse); 363067 (Rat)
Alternate Names
DKFZp434K2235; FLJ90319; Histone deacetylase complex subunit Sin3a; KIAA0700; paired amphipathic helix protein Sin3a; SIN3 homolog A, transcription regulator (yeast); SIN3 homolog A, transcriptional regulator (yeast); SIN3A; Transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; transcriptional regulator, SIN3A

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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