Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 906552
View all Somatostatin Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Somatostatin peptide
Accession # P61278

Specificity

Detects human Somatostatin in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human/Mouse Somatostatin Antibody

Somatostatin antibody in Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Somatostatin in Human Embryonic Stem Cells.

Somatostatin was detected in immersion fixed human embryonic stem cells differentiated to pancreatic cells using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Somatostatin antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Somatostatin in beta TC‑6 Mouse Cell Line.

Somatostatin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Somatostatin antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Somatostatin in Human Pancreas.

Somatostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Somatostatin in TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative).

Somatostatin was detected in immersion fixed TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunofluorescent Staining of iPSC-derived Beta Islets.

iPSC-derived beta islets were fixed, frozen, and sectioned. Sections were stained with a Mouse Anti-Human C-peptide Monoclonal Antibody (Catalog # MAB14171), and a Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358), followed by secondary antibody staining with the NorthernLights NL493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009; green) and NorthernLights NL557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013; red). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid.

Applications for Human/Mouse Somatostatin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human embryonic stem cells differentiated into pancreatic cells and beta TC‑6 mouse beta cell insulinoma cell line, TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 2 reviews rated 5 using MAB2358 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Somatostatin

Somatostatin (SST), also known as SMST, is a 116 amino acid (aa) peptide hormone protein and shares 96% aa identity with mouse and rat SST. It exists as a preproprotein that is cleaved into a proprotein form and then subsequently into 14 aa and 28 aa active peptide forms. SST is secreted by many tissues throughout the body and inhibits the secretion of many other hormones. Some of its major roles are inhibiting growth hormone secretion by the pituitary gland, insulin and glucagon secretion in pancreatic islets, and secretion of gastrointestinal tract hormones such as gastrin. Additionally, SST has been shown to have neuromodulatory activity.

Alternate Names

SMST, SOM, SRIF, SST

Entrez Gene IDs

6750 (Human); 20604 (Mouse); 24797 (Rat)

Gene Symbol

SST

UniProt

P61278

Additional Somatostatin Products

Product Documents for Human/Mouse Somatostatin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Somatostatin Antibody

For research use only

Citations for Human/Mouse Somatostatin Antibody

Customer Reviews for Human/Mouse Somatostatin Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
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1 Stars
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  • Human/Mouse Somatostatin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Mouse
    Verified Customer | Posted 11/09/2021
    Human/Mouse Somatostatin Antibody MAB2358
  • Human/Mouse Somatostatin Antibody
    Name: Ruslan Rust
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 04/05/2018
    Somatostatin is shown in green. Parvalbumin is shown in purple. NeuN is shown in white.
    Human/Mouse Somatostatin Antibody MAB2358

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Protocols

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