Human/Mouse SUMO2/3/4 Antibody

Catalog # Availability Size / Price Qty
AF3020
AF3020-SP
Human/Mouse SUMO2/3/4 Antibody in Western Blot
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Human/Mouse SUMO2/3/4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse proteins modified with SUMO2, 3, or 4 in Western blots. Because of the high level of sequence homology, this antibody has equivalent reactivity to SUMO2, SUMO3, and SUMO4 based in Western blots with recombinant SUMO protein. Cross-reactivity to recombinant human SUMO1 was minimal in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SUMO2
Ala2-Tyr95
Accession # P61956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SUMO2/3/4 by Western Blot. View Larger

Detection of Human SUMO2/3/4 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse SUMO2/3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3020) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SUMO2/3/4 at approximately 12 kDa (as indicated) along with multiple sumoylated proteins. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUMO2/3/4

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. Unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All SUMO proteins share the conserved ubiquitin domain and the C-terminal diglycine cleavage/attachment site. Human SUMO2, also known as Sentrin2 and SMT3B is synthesized as a 95 amino acid (aa), 11 kDa propeptide that contains a two aa C-terminal prosegment, and an 18 aa N-terminal protein interacting region (aa 33‑50). Following prosegment cleavage, the C-terminal glycine is enzymatically attached to a lysine on a target protein. Human SUMO2 shares 100% sequence identity to SUMO-2 from mouse. SUMO2 also has very high sequence homology to SUMO3 and SUMO4, 86 % and 85%, respectively. SUMO2 shares only 44% sequence identity to SUMO1.

Long Name
Small Ubiquitin-like Modifier 2, 3, & 4
Alternate Names
SUMO2/3/4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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