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Human/Mouse TREM2 Antibody

R&D Systems | Catalog # MAB17291

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Knockout Validated, ELISA, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, CyTof, ELISA Capture, ELISA Detection, ELISA Development (Capture), In vivo assay, Functional Assay, Functional Assay Control, Receptor Recycling

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 237920
View all TREM2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM2
extracellular domain
Accession # Q99NH8

Specificity

Detects human and mouse TREM2 in direct ELISAs. Stains TREM2 transfectants but not TREM1 transfectants in flow cytometry. In sandwich ELISAs, detects mouse TREM2 when paired with a mouse TREM2 detection antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse TREM2 Antibody

Detection of TREM2 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of TREM2 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0113).

Detection of TREM2 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of TREM2 in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).

Detection of TREM2 antibody in Human Mature Dendritic Cells antibody by Flow Cytometry.

Detection of TREM2 in Human Mature Dendritic Cells by Flow Cytometry.

Human mature dendritic cells were stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).

Detection of TREM2 antibody in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM2 or Human TREM1 antibody by Flow Cytometry.

Detection of TREM2 in HEK293 Human Cell Line Transfected with eGFP and Either Human TREM2 or Human TREM1 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with eGFP and either (A) human TREM2 or (B) human TREM1 was stained with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061).

TREM2 Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line by Immunocytochemistry (ICC).

TREM2 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line but is not detected in TREM2 knockout (KO) RAW 264.7 Mouse Cell Line cell line using Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips.

Mouse TREM2 Antibody in ELISA Standard Curve.

Mouse TREM2 ELISA Standard Curve.

Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of TREM2 in PBMC monocytes by Flow Cytometry

PBMC monocytes were stained with Mouse Anti-Human CD11b/Integrin alpha M PE‑conjugated Monoclonal Antibody (Catalog # FAB16991P) and either (A) Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) or (B) isotype control antibody (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse TREM2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as a mouse TREM2 ELISA capture antibody when paired with Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line, THP1 human acute monocytic leukemia cell line, human mature dendritic cells, and HEK293 human embryonic kidney cell line transfected with eGFP and either human TREM2 or human TREM1; human PBMC monocytes

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line

Knockout Validated

TREM2 was detected in immersion fixed RAW 264.7 mouse cell line but is not detected TREM2 knockout (KO) RAW 264.7 mouse cell line.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB17291 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM2

TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.

Long Name

Triggering Receptor Expressed on Myeloid Cells 2

Alternate Names

PLOSL2, TREM-2

Entrez Gene IDs

54209 (Human); 102133279 (Cynomolgus Monkey)

Gene Symbol

TREM2

UniProt

Q99NH8 (Mouse)

Additional TREM2 Products

Product Documents for Human/Mouse TREM2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse TREM2 Antibody

For research use only

Citations for Human/Mouse TREM2 Antibody

Customer Reviews for Human/Mouse TREM2 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • TREM2 staining in AD mouse model
    Name: Cody Zurhellen
    Application: Immunohistochemistry
    Sample Tested: Brain (cortex) tissue
    Species: Mouse
    Verified Customer | Posted 12/02/2024
    AD Mouse model (Hippocampus)
    TREM2 staining performed on AD mouse model to reveal activated subset of microglia specifically around plaques. Worked well using both IHC and IF staining methods.
    Human/Mouse TREM2 Antibody MAB17291
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human/Mouse TREM-2 Antibody
    Name: Leslie Priddy
    Application: Immunohistochemistry
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 04/13/2018
  • Human/Mouse TREM-2 Antibody
    Name: Ju Shi
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/01/2017

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Protocols

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FAQs for Human/Mouse TREM2 Antibody

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    • Q: What type of light chain does the Human/Mouse TREM-2 Antibody, Cat# MAB17291, clone # 237920 have?
      A: This antibody has a kappa light chain.
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