Human/Mouse UCP2 Antibody

Detection of Human/Mouse UCP2 by Western Blot. Western blot shows lysates of mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human UCP1, UCP2, UCP3, and UCP4 (5 ng/lane) were included. A specific band for UCP2 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Detection of Mouse UCP2 by Simple Western^TM. Simple Western lane view shows lysates of mouse brown adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for UCP2 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of UCP2 by Western Blot Adiponectin enhances the mRNA stability of UCP2 and promotes its protein synthesis.Two inhibitors, actinomycin D (ActD, A, B and C) or cycloheximide (CHX, A, B and D), were administered together with or without adiponectin protein into liver tissues of AKO mice livers. The protein (A) and mRNA (B) abundance of UCP2 was monitored by Western blotting and QPCR, respectively. The relative protein expression was also monitored in PCs/NPCs lysates (C and D). *, P<0.05 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of UCP2 by Western Blot Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of UCP2 by Western Blot Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of UCP2 by Western Blot Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. The NPCs were used for further fractionation to collect those enriched with Kupffer (K)- and sinusoidal endothelial (E) cells. The enrichment of the two cell types were confirmed by Western blotting using macrophage marker F4/80 and sinusoidal endothelial marker SE-1, respectively (A). UCP2 expression was monitored as in Figure 1. After densitometry analysis, the protein ratio of UCP2/ beta -actin was calculated and presented as fold changes against Luci Kupffer samples (B). UCP2 gene expression was also quantified in four types of cells treated with or without adiponectin (10 µg/ml) (C). *, P<0.05 and **, P<0.01 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.