Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UCP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCP2
Met1-Phe309
Accession # P55851

Specificity

Detects human and mouse UCP2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) UCP1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse UCP2 Antibody

Detection of Human/Mouse UCP2 antibody by Western Blot.

Detection of Human/Mouse UCP2 by Western Blot.

Western blot shows lysates of mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human UCP1, UCP2, UCP3, and UCP4 (5 ng/lane) were included. A specific band for UCP2 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Detection of Mouse UCP2 antibody by Simple WesternTM.

Detection of Mouse UCP2 by Simple WesternTM.

Simple Western lane view shows lysates of mouse brown adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for UCP2 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse UCP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4739) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of UCP2 by Western Blot

Detection of UCP2 by Western Blot

Adiponectin enhances the mRNA stability of UCP2 and promotes its protein synthesis.Two inhibitors, actinomycin D (ActD, A, B and C) or cycloheximide (CHX, A, B and D), were administered together with or without adiponectin protein into liver tissues of AKO mice livers. The protein (A) and mRNA (B) abundance of UCP2 was monitored by Western blotting and QPCR, respectively. The relative protein expression was also monitored in PCs/NPCs lysates (C and D). *, P<0.05 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of UCP2 by Western Blot

Detection of UCP2 by Western Blot

Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of UCP2 by Western Blot

Detection of UCP2 by Western Blot

Acute treatment with adiponectin elevates UCP2 expression in NPCs.PBS or 50 µg of murine adiponectin protein was injected into the portal vein of AKO mice livers. Liver tissues were collected for evaluation of UCP2 expression. The protein abundance in mitochondria (A), mRNA expression in total tissue lysates (B), and the protein content in PC and NPC fractions (C) were analyzed as in Figure 1. *, P<0.05 vs vehicle treated samples, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of UCP2 by Western Blot

Detection of UCP2 by Western Blot

Adiponectin promotes UCP2 expression in hepatic endothelial cells.AKO mice were treated as in Figure 1B. The NPCs were used for further fractionation to collect those enriched with Kupffer (K)- and sinusoidal endothelial (E) cells. The enrichment of the two cell types were confirmed by Western blotting using macrophage marker F4/80 and sinusoidal endothelial marker SE-1, respectively (A). UCP2 expression was monitored as in Figure 1. After densitometry analysis, the protein ratio of UCP2/ beta -actin was calculated and presented as fold changes against Luci Kupffer samples (B). UCP2 gene expression was also quantified in four types of cells treated with or without adiponectin (10 µg/ml) (C). *, P<0.05 and **, P<0.01 vs corresponding controls, n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22359684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse UCP2 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Mouse brown adipose tissue

Western Blot

1 µg/mL
Sample: Mouse brown adipose tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UCP2

Mitochondrial Uncoupling Protein 2 (UCP2) is a 33 kDa member of the mitochondrial carrier protein family. UCP2 uncouples respiration from oxidative phosphorylation, principally in fat and skeletal muscle. Unlike UCP1, which generates heat in response to cold, UCP2 generates heat in response to dietary fluctuations. Human UCP2 is 309 amino acid (aa) in length. It contains six transmembrane domains (aa’s 11‑291) and is embedded in the inner mitochondrial membrane. Here, it dimerizes, forming a proton channel. There is one nucleotide binding site (aa 276‑298). Full‑length UCP2 shares 94%, 97% and 96% aa identity with porcine, canine and mouse UCP2, respectively.

Long Name

Uncoupling Protein 2

Alternate Names

SLC25A8, UCPH

Entrez Gene IDs

7351 (Human); 22228 (Mouse); 54315 (Rat)

Gene Symbol

UCP2

UniProt

P55851

Additional UCP2 Products

Product Documents for Human/Mouse UCP2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse UCP2 Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse UCP2 Antibody

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