Wnt-3a Antibody (217804.2R)

R&D Systems | Catalog # MAB13242

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 217804.2R
View all Wnt-3a Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant mouse Wnt-3a
Ser36-Gln75, Trp219-Arg269
Accession # P27467

Specificity

Detects mouse Wnt-3a in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Wnt-3a Antibody (217804.2R)

Detection of Mouse Wnt-3a antibody by Western Blot.

Detection of Mouse Wnt‑3a by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse Wnt-3a. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-3a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Wnt3A antibody in BG01V Human Stem Cells antibody by Flow Cytometry.

Detection of Wnt3A in BG01V Human Stem Cells by Flow Cytometry.

BGO1v human embryonic stem cells were stained with Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Wnt-3a Antibody (217804.2R)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/mL
Sample: BGO1v human embryonic stem cells fixed and permeabilized with  FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Western Blot

1 µg/mL
Sample: CHO Chinese hamster ovary cell line transfected with mouse Wnt-3a

Reviewed Applications

Read 1 review rated 4 using MAB13242 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

References

  1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
  2. Miller, J.R. (2001) Genome Biol. 3:3001.
  3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  4. Lu, W et al. (2004) Cell 119:97.
  5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
  6. Willert, K. et al. (2003) Nature 423:448.
  7. Komekado, H. et al. (2007) Genes Cells 12:521.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Entrez Accession # NP_001100475.
  10. Dunty, W.C. et al. (2008) Development 135:85.
  11. Lee, S.M.K. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  14. Nusse, R. (2008) Cell Res. 18:523.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Entrez Gene IDs

89780 (Human); 22416 (Mouse)

Gene Symbol

WNT3A

UniProt

P27467

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody (217804.2R)

Certificate of Analysis

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Product Specific Notices for Wnt-3a Antibody (217804.2R)

For research use only

Citations for Wnt-3a Antibody (217804.2R)

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  • Human/Mouse Wnt-3a Antibody
    Name: Anonymous
    Application: Western Blot
    Verified Customer | Posted 01/19/2024
    This antibody demonstrates effective performance in ELISA detection, and we have successfully applied it in Western blot experiments. Additionally, it has shown reliable results across various cancer cell groups.
    Wnt-3a Antibody (217804.2R) MAB13242

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