Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # QCRL
View all MRP1 Primary Antibodies »

Product Specifications

Immunogen

H69AR human small cell lung cancer cell line membrane extracts

Specificity

Detects human MRP1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MRP1 Antibody

MRP1 in Human PBMCs.

MRP1 was detected in immersion fixed Human peripheral blood mononuclear cells using Mouse Anti-Human MRP1 Monoclonal Antibody (Catalog # MAB19291) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human MRP1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Human peripheral blood mononuclear cells

Immunoprecipitation

Hipfner, D.R., et al. (1994) Canc. Res. 54:5788.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: H69AR human small cell lung cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

Hipfner, D.R. et al. (1994) Canc. Res. 54:5788; Hipfner, D.R. et al. (1996) Canc. Res. 56:3307. This application was not tested by R&D Systems.

Reviewed Applications

Read 1 review rated 5 using MAB19291 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MRP1

MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.

Long Name

Multi-drug Resistance Protein 1

Alternate Names

ABCC1, GS-X

Entrez Gene IDs

4363 (Human); 17250 (Mouse); 24565 (Rat)

Gene Symbol

ABCC1

Additional MRP1 Products

Product Documents for Human MRP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MRP1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human MRP1 Antibody

Customer Reviews for Human MRP1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human MRP1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human MRP1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 04/06/2022
    Tissue was cyropreserved
    Human MRP1 Antibody MAB19291

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies