Human MSH6 Antibody (1128063)

MSH6 in Human Colon Tumor via seqIF™ staining on COMET™

MSH6 was detected in immersion fixed paraffin-embedded sections of human colon tumor using Mouse Anti-Human MSH6, Monoclonal Antibody (Catalog # MAB11832) at 25ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of Human MSH6 by Western Blot.

Western Blot shows lysates of HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human MSH6 Monoclonal Antibody (Catalog # MAB11832) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MSH6 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human MSH6 by Simple Western^TM.

Left: Simple Western lane view shows lysates of HCT‑116 human colorectal carcinoma cell line, loaded at 0.1 mg/ml. A specific band was detected for MSH6 at approximately 165 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Mouse Anti-Human MSH6 Monoclonal Antibody (Catalog # MAB11832) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MSH6 Monoclonal Antibody (Catalog # MAB11832) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HCT‑116 human colorectal carcinoma cell line.