Detection of Human Myocilin by Western Blot.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Myocilin by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa,
secreted polypeptide that belongs to the family of olfactomedin-related
proteins. Human Myocilin is synthesized as a 490 amino acid (aa)
precursor that contains an 18 aa signal sequence, an 84 aa N-terminus,
a 53 aa alpha -helical leucine zipper and a 335 aa C-terminus that contains
a 260 aa OLF-domain. An alternate start site generates a
signal-sequenceless 504 aa mature protein. Myocilin forms
nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain
shares 87% aa sequence identity with the OLF-domain in bovine, rat and
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