Human Napsin A Antibody

R&D Systems | Catalog # AF8489

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Napsin A Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
Thr25-Gly420
Accession # O96009

Specificity

Detects human Napsin A in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Napsin A Antibody

Detection of Human Napsin A antibody by Western Blot.

Detection of Human Napsin A by Western Blot.

Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Napsin A antibody in Human Kidney by Immunohistochemistry (IHC-P).

Napsin A in Human Kidney.

Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Napsin A by Simple WesternTM.

Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 45 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Napsin A Antibody

Application
Recommended Usage

Immunohistochemistry

0.5-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

0.5 µg/mL
Sample: Human Kidney

Western Blot

1:1000 dilution
Sample: Human lung tissue and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Napsin A

Novel Aspartic Proteinase of the Pepsin Family or Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It consists of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Entrez Gene IDs

9476 (Human); 16541 (Mouse); 60575 (Rat)

Gene Symbol

NAPSA

UniProt

O96009

Additional Napsin A Products

Product Documents for Human Napsin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Napsin A Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Related Research Areas

Cancer Biomarkers
Lysosomal Enzymes

Citations for Human Napsin A Antibody

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Protocols

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