Human Napsin A Antibody

Catalog # Availability Size / Price Qty
AF8489
AF8489-SP
Detection of Human Napsin A by Western Blot.
3 Images
Product Details
Citations (1)
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Human Napsin A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Napsin A in direct ELISAs and Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
Thr25-Gly420
Accession # O96009
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Simple Western
0.5 µg/mL
Human Kidney
Immunohistochemistry
0.5-10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Napsin A antibody by Western Blot. View Larger

Detection of Human Napsin A by Western Blot. Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Napsin A antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Napsin A in Human Kidney. Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Napsin A by Simple WesternTM. Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 45 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Napsin A

Novel Aspartic Proteinase of the Pepsin Family or Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It consists of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.

Entrez Gene IDs
9476 (Human); 16541 (Mouse); 60575 (Rat)
Alternate Names
Asp 4; ASP4; Aspartyl protease 4; EC 3.4.23; EC 3.4.23.-; EC 3.4.23.15; EC 3.4.23.3; EC 3.4.23.5; KAP; Kdap; NAP1napsin-A; NAPA; NAPApronapsin A; NAPSA; napsin A aspartic peptidase; Napsin A; Napsin-1; SNAPA; TA01/TA02

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Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citation for Human Napsin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Placenta Derived C-Terminal Fragment of &beta-Hemoglobin With Combined Antibacterial and Antiviral Activity
    Authors: R Gro beta, R Bauer, F Krüger, E Rücker-Bra, LR Olari, L Ständker, N Preising, AA Rodríguez, C Conzelmann, F Gerbl, D Sauter, F Kirchhoff, B Hagemann, J Ga?anin, T Weil, YB Ruiz-Blanc, E Sanchez-Ga, WG Forssmann, A Mankertz, S Santibanez, S Stenger, P Walther, S Wiese, B Spellerber, J Münch
    Front Microbiol, 2020;11(0):508.
    Species: Human
    Sample Types: Tissue
    Applications: Dot Blot

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