Human Napsin A Antibody Summary
Accession # O96009
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Napsin A by Western Blot. Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Napsin A in Human Kidney. Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Napsin A by Simple WesternTM. Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 45 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Napsin A
Novel Aspartic Proteinase of the Pepsin Family or Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It consists of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.
Product Specific Notices* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
Citation for Human Napsin A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A Placenta Derived C-Terminal Fragment of &beta-Hemoglobin With Combined Antibacterial and Antiviral Activity
Authors: R Gro beta, R Bauer, F Krüger, E Rücker-Bra, LR Olari, L Ständker, N Preising, AA Rodríguez, C Conzelmann, F Gerbl, D Sauter, F Kirchhoff, B Hagemann, J Ga?anin, T Weil, YB Ruiz-Blanc, E Sanchez-Ga, WG Forssmann, A Mankertz, S Santibanez, S Stenger, P Walther, S Wiese, B Spellerber, J Münch
Front Microbiol, 2020;11(0):508.
Sample Types: Tissue
Applications: Dot Blot
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