Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Neprilysin-2/MMEL1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2/MMEL1
Gly69-Trp770
Accession # AAL08942

Specificity

Detects human Neprilysin-2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Neprilysin‑2/MMEL1 Antibody

Detection of Neprilysin‑2/MMEL1 in NS0 transfectant cells by Flow Cytometry

NS0 cells transfected with hMMEL1 were stained with Goat Anti-Human Neprilysin‑2/MMEL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2340, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Neprilysin‑2/MMEL1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 cells transfected with hMMEL1

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)

Reviewed Applications

Read 1 review rated 4 using AF2340 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neprilysin-2/MMEL1

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

References

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.

Alternate Names

MMEL1, MMEL2, Neprilysin2, NL2, SEP

Entrez Gene IDs

79258 (Human)

Gene Symbol

MMEL1

UniProt

AAL08942

Additional Neprilysin-2/MMEL1 Products

Product Documents for Human Neprilysin‑2/MMEL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Neprilysin‑2/MMEL1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Neprilysin‑2/MMEL1 Antibody

Customer Reviews for Human Neprilysin‑2/MMEL1 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22008264
    Species: Human
    Verified Customer | Posted 01/06/2015

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Protocols

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