Human Neuromedin BR/NMBR Antibody (466505)

R&D Systems | Catalog # MAB4728

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 466505
View all Neuromedin BR/NMBR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human NMBR
Met1-Met390
Accession # AAB27330

Specificity

Detects human Neuromedin B R/NMBR. Stains human Neuromedin B R/NMBR transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Neuromedin BR/NMBR Antibody (466505)

Detection of Neuromedin B R/ NMBR antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of Neuromedin B R/ NMBR in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Neuromedin B R/NMBR antibody in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP antibody by Flow Cytometry.

Detection of Neuromedin B R/NMBR in HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) Neuromedin B R/NMBR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Neuromedin B R/NMBR Monoclonal Antibody (Catalog # MAB4728) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Neuromedin BR/NMBR Antibody (466505)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Neuromedin B R/NMBR and eGFP

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuromedin BR/NMBR

Neuromedin B Receptor (NMBR) is an 80 kDa, 390 amino acid (aa) G-protein coupled 7-transmembrane glycoprotein receptor for bombesin-like peptides, binding Neuromedin B with high affinity and GRP (gastrin releasing peptide) with lower affinity.  Neuromedin B R expression in the olfactory and central thalamic regions of the brain plays a role in thermoregulation. It has also been shown to be mitogenic in colonic epithelium. Expression in the pituitary gland is important for regulation of the pituitary-thyroid axis. Within extracellular domains, human Neuromedin B R shares 86% and 82% aa identity with mouse and rat Neuromedin B R, respectively.

Long Name

Neuromedin B Receptor

Alternate Names

Neuromedin B R, NMBR

Entrez Gene IDs

4829 (Human); 18101 (Mouse); 25264 (Rat)

Gene Symbol

NMBR

UniProt

AAB27330

Additional Neuromedin BR/NMBR Products

Product Documents for Human Neuromedin BR/NMBR Antibody (466505)

Certificate of Analysis

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Product Specific Notices for Human Neuromedin BR/NMBR Antibody (466505)

For research use only

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