Human Neuronostatin Antibody

Detection of Neuronostatin in TT cells (Positive) and THP-1 cells (Negative). Neuronostatin was detected in fixed TT human medullary thyroid cancer cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Neuronostatin in Human Pancreas. Neuronostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Neuronostatin by Simple Western^TM. Simple Western shows lysates of Neuronostatin Peptide SML0354, loaded at 200 ng/ml. Specific bands were detected for Neuronostatin at approximately 2-4 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.