Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2956E
View all Neuronostatin Primary Antibodies »

Product Specifications

Immunogen

Human Neuronostatin containing synthetic peptide

Specificity

Detects a peptide specific for Neuronostatin in a region around amino acid 95 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Neuronostatin Antibody

Detection of Neuronostatin in TT cells (Positive) and THP-1 cells (Negative).

Neuronostatin was detected in fixed TT human medullary thyroid cancer cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Neuronostatin in Human Pancreas.

Neuronostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Neuronostatin by Simple WesternTM.

Simple Western shows lysates of Neuronostatin Peptide SML0354, loaded at 200 ng/ml. Specific bands were detected for Neuronostatin at approximately 2-4 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human Neuronostatin Antibody

Application
Recommended Usage

Immunocytochemistry

1-10 µg/mL
Sample: fixed TT human medullary thyroid cancer cell line (Positive) and absent in THP-1 human acute monocytic leukemia cell line (Negative)

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

20 µg/mL
Sample: Neuronostatin peptide SML0354

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuronostatin

Neuronostatin is a 13-amino acid bioactive peptide hormone encoded by the somatostatin gene.  Neuronostatin has a widespread effect in the CNS and peripheral tissues.  It reduces food and water intake, increases mean arterial pressure, delays gastrointestinal transit, and increases glucagon secretion from pancreatic islet alpha cells among other things.  Neuronostatin is produced in the endocrine pancreas, specifically pancreatic beta cells. The amounts of immunoreactive neuronostatin are highest in spleen, pancreas, cerebrum, and hypothalamus but is also found in diverse tissues including the heart.

References

  1. Yang S, Zhao X, Du Y, Yu P. Emerging functions of neuronostatin in physiology, pathology, and potential therapeutics. Neuropeptides. 2022 Aug;94:102257. doi: 10.1016/j.npep.2022.102257. Epub 2022 May 20. PMID: 35660860.
  2. Wei D, Sun Y. Krążek M, Ligęza A, Wojciechowicz T, Skrzypski M. Biologiczna rola neuronostatyny, hormonu kodowanego przez gen somatostatyny [The biological role of neuronostatin, a hormone encoded by the somatostatin gene]. Postepy Biochem. 2023 Jun 5;69(2):89-95. Polish. doi: 10.18388/pb.2021_476. PMID: 37493559. 
  3. Salvatori AS, Elrick MM, Samson WK, Corbett JA, Yosten GL. Neuronostatin inhibits glucose-stimulated insulin secretion via direct action on the pancreatic alpha -cell. Am J Physiol Endocrinol Metab. 2014 Jun 1;306(11):E1257-63. doi: 10.1152/ajpendo.00599.2013. Epub 2014 Apr 15. PMID: 24735892; PMCID: PMC4042099.
  4. Vainio L, Perjes A, Ryti N, Magga J, Alakoski T, Serpi R, Kaikkonen L, Piuhola J, Szokodi I, Ruskoaho H, Kerkelä R. Neuronostatin, a novel peptide encoded by somatostatin gene, regulates cardiac contractile function and cardiomyocyte survival. J Biol Chem. 2012 Feb 10;287(7):4572-80. doi: 10.1074/jbc.M111.289215. Epub 2011 Dec 14. PMID: 22170057; PMCID: PMC3281621.

Long Name

Neuronostatin

Alternate Names

Neuronostatin-13

Entrez Gene IDs

6750 (Human); 20604 (Mouse); 24797 (Rat)

Gene Symbol

P61278

Additional Neuronostatin Products

Product Documents for Human Neuronostatin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Neuronostatin Antibody

For research use only

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Protocols

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