Neuropilin-1 (NRP1) is a transmembrane glycoprotein that regulates axon guidance and angiogenesis. Distinct Neuropilin-1 domains mediate its binding to heparin, the heparin-binding forms of VEGF and PlGF (PlGF2), and Semaphorin 3. Neuropilin-1 can form homo- and hetero-oligomers with Neuropilin-2. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. They mediate axon repulsion on sensory and sympathetic neurons through binding to Class III Semaphorins, Plexin A3, and Plexin A4. They also associate with VEGF R2 and function as coreceptors for VEGF. NRP1 is preferentially expressed in developing or remodeling arteries, epithelial cells, and carcinomas, while Neuropilin-2 is primarily found in veins and lymphatics. Neuroplin-1 is additionally expressed on dendritic cells where it mediates DC-induced T cell proliferation. NRP1 also plays a role in SARS-CoV-2 infection in humans, the virus responsible for COVID-19. The Spike protein of SARS-CoV-2 can bind to the b1 domain of Neuropilin-1 on the cell surface initiating viral entry into the host cell. Blocking the NRP1-Spike interaction reduces viral infectivity in vitro making it a potential therapeutic target.
Human Neuropilin-1 DuoSet ELISA
R&D Systems | Catalog # DY3870-05
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human Neuropilin-1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Neuropilin-1 DuoSet ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Scientific Data Images for Human Neuropilin-1 DuoSet ELISA
Human Neuropilin-1 ELISA Standard Curve
Kit Contents for Human Neuropilin-1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
Stability & Storage
Background: Neuropilin-1
Alternate Names
Gene Symbol
Additional Neuropilin-1 Products
Product Documents for Human Neuropilin-1 DuoSet ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Neuropilin-1 DuoSet ELISA
For research use only
Related Research Areas
Citations for Human Neuropilin-1 DuoSet ELISA
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
VEGF - VEGF R2 Signaling Pathways
