Human Neuropilin-1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY3870-05
Ancillary Products Available
Human Neuropilin-1 ELISA Standard Curve
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human Neuropilin-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Neuropilin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Neuropilin-1 ELISA Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously known as neuropilin) and Neuropilin-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind distinct members of the class III secreted semaphorin subfamily that are implicated in repulsive axon guidance. Neuropilin extracellular domains contain two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. In the absence of ligands, neuropilins can form homo- and hetero-oligomers via their MAM domains. Expression in developing neurons of the central and peripheral nervous systems is somewhat overlapping but distinct. Npn-1 and Npn-2 are also receptors for VEGF165 on endothelial and tumor cells.

Entrez Gene IDs:
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names:
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

Citations for Human Neuropilin-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Evaluating circulating soluble markers of endothelial dysfunction and risk factors associated with PE: A multicentre longitudinal case control study in northern Ghana
    Authors: Ahenkorah, B;Sakyi, SA;Fondjo, LA;Helegbe, G;Owiredu, EW;Der, EM;Amoah, LE;Kusi, KA;Obiri, D;Amoani, B;Bimpong, S;Ofosu, W;Obirikorang, C;Odame, E;Larbie, C;Arthur-Johnson, P;Quaye, I;Ametefe, EM;Okai, BK;Anormah, R;Akorli, E;Simono Charadan, AM;Aboubacar, RM;Amesewu, E;Gyan, B;
    Heliyon
    Species: Human
    Sample Types: Serum
  2. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022-07-26;15(1):87.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Dysregulated Tear Fluid Nociception-Associated Factors, Corneal Dendritic Cell Density, and Vitamin D Levels in Evaporative Dry Eye
    Authors: P Khamar, AP Nair, R Shetty, T Vaidya, M Subramani, M Ponnalagu, K Dhamodaran, S D'souza, A Ghosh, N Pahuja, R Deshmukh, P Ahuja, K Sainani, RMMA Nuijts, D Das, A Ghosh, S Sethu
    Invest. Ophthalmol. Vis. Sci., 2019-06-03;60(7):2532-2542.
    Species: Human
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human Neuropilin-1 DuoSet ELISA

There are currently no reviews for this product. Be the first to review Human Neuropilin-1 DuoSet ELISA and earn rewards!

Have you used Human Neuropilin-1 DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review