Human NF-L Antibody

Catalog # Availability Size / Price Qty
MAB22162-100
MAB22162-SP
NF‑L in U‑251 MG Human Cell Line.
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Human NF-L Antibody Summary

Species Reactivity
Human
Specificity
Detects human NF-L in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 985917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Confirmation of Specificity using RNAscope ISH
Immersion fixed U-251 MG human glioblastoma cell line
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry View Larger

NF‑L in U‑251 MG Human Cell Line. NF‑L was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human NF‑L Monoclonal Antibody (Catalog # MAB22162) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry View Larger

NF-L in U-251 MG human glioblastoma cell line using RNAscope®ISH NF-L mRNA was detected in formalin-fixed U-251 MG human glioblastoma cell line probed with ACD RNAScope®Probe-Hs-NEFL (Catalog # 468671) and stained using ACD RNAscope®2.5 HD Detection Reagents-RED (Catalog # 32260). This is an In Situ Hybridization control for NF-L expression.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name
Neurofilament Protein, Light Chain
Entrez Gene IDs
4747 (Human)
Alternate Names
68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NF-L Antibody

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Human NF-L Antibody
By Anonymous on 12/03/2021
Application: ICC/IF Sample Tested: fibroblasts Species: Human