Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 714401
View all NFIL3/E4BP4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFIL3/E4BP4
Lys140-Gly462
Accession # Q16649

Specificity

Detects human NFIL3/E4BP4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NFIL3/E4BP4 Antibody

Detection of NFIL3/E4BP4 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of NFIL3/E4BP4 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8570) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Human NFIL3/E4BP4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

References

  1. Seillet C, et al (2014) J Exp Med. 211:1733.
  2. Geiger TL, et al (2014) J Exp Med. 211:1723.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Alternate Names

E4BP4, IL3BP1, NF-IL3A

Entrez Gene IDs

4783 (Human); 18030 (Mouse); 114519 (Rat)

Gene Symbol

NFIL3

UniProt

Q16649

Additional NFIL3/E4BP4 Products

Product Documents for Human NFIL3/E4BP4 Antibody

Certificate of Analysis

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Product Specific Notices for Human NFIL3/E4BP4 Antibody

For research use only

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