Human Niemann-Pick Type C2/NPC2 Antibody

Catalog # Availability Size / Price Qty
AF8644
AF8644-SP
Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot.
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Human Niemann-Pick Type C2/NPC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
Glu20-Leu151
Accession # P61916
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Immunohistochemistry
1:50 dilution
See below
Immunocytochemistry
1:150 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot. View Larger

Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line. View Larger

Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line. Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Niemann-Pick Type C2/NPC2 in Human Kidney. View Larger

Niemann-Pick Type C2/NPC2 in Human Kidney. Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Background: Niemann-Pick Type C2/NPC2

Entrez Gene IDs
10577 (Human); 67963 (Mouse); 286898 (Rat)
Alternate Names
EDDM1; Epididymal Protein 1; epididymal secretory protein E1; HE1; HE1epididymal protein 1; Human epididymis-specific protein 1; MGC1333; Niemann-Pick disease type C2 protein; Niemann-Pick disease, type C2; NiemannPick Type C2; Niemann-Pick Type C2; NPC2; NP-C2; tissue-specific secretory protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

Reviews for Human Niemann-Pick Type C2/NPC2 Antibody

Average Rating: 4 (Based on 1 Review)

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Human Niemann-Pick Type C2/NPC2 Antibody
By Anonymous on 12/28/2017
Application: IHC Sample Tested: Skin tissue Species: Human