Human Niemann-Pick Type C2/NPC2 Antibody

R&D Systems | Catalog # AF8644

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Niemann-Pick Type C2/NPC2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Niemann-Pick Type C2/NPC2
Glu20-Leu151
Accession # P61916

Specificity

Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Niemann-Pick Type C2/NPC2 Antibody

Detection of Human Niemann-Pick Type C2/NPC2 antibody by Western Blot.

Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Niemann-Pick Type C2/NPC2 antibody in SK-Hep-1 Human Cell Line by Immunocytochemistry (ICC).

Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Niemann-Pick Type C2/NPC2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

Niemann-Pick Type C2/NPC2 in Human Kidney.

Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Niemann-Pick Type C2/NPC2 Antibody

Application
Recommended Usage

Immunocytochemistry

1:150 dilution
Sample: Immersion fixed SK-Hep-1 human liver adenocarcinoma cell line

Immunohistochemistry

1:50 dilution
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1:1000 dilution
Sample: NTera‑2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using AF8644 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

  • 12 months from date of receipt, -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Niemann-Pick Type C2/NPC2

Niemann-Pick type C2 encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Alternate Names

EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2

Entrez Gene IDs

10577 (Human); 67963 (Mouse); 286898 (Rat)

Gene Symbol

NPC2

UniProt

P61916

Additional Niemann-Pick Type C2/NPC2 Products

Product Documents for Human Niemann-Pick Type C2/NPC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Niemann-Pick Type C2/NPC2 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Citations for Human Niemann-Pick Type C2/NPC2 Antibody

Customer Reviews for Human Niemann-Pick Type C2/NPC2 Antibody (1)

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  • Human Niemann-Pick Type C2/NPC2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 12/28/2017
    Human Niemann-Pick Type C2/NPC2 Antibody AF8644

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Protocols

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