Human NLRP3/NALP3 Antibody Summary
Accession # Q96P20
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
NLRP3/NALP3 in Jurkat Human Cell Line. NLRP3/NALP3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NLRP3 (NAcht Leucine-rich repeat Protein 3), also known as NALP3, CIAS1, PYPAF or Cryopyrin is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NLRP3 contains an N-terminal Pyrin domain (aa 1-93) followed by a Nacht region (aa 220-536), and seven LRRs (aa 740-991). Within the sequence used as an immunogen, human NLRP3 shares 76% and 75% aa identity with mouse and rat NLRP3, respectively. Isoforms of 979, 979, 922 and 719 aa lack aa 720–776, aa 836-892, both regions, or terminate at aa 719.
Citation for Human NLRP3/NALP3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Mitochondrial NLRP3 protein induces reactive oxygen species to promote Smad protein signaling and fibrosis independent from the inflammasome.
Authors: Bracey N, Gershkovich B, Chun J, Vilaysane A, Meijndert H, Wright J, Fedak P, Beck P, Muruve D, Duff H
J Biol Chem, 2014;289(28):19571-84.
Sample Types: Whole Tissue
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