Human NLRP3/NALP3 Antibody

Catalog # Availability Size / Price Qty
NLRP3/NALP3 in Jurkat Human Cell Line.
1 Image
Product Details
Citations (1)
Supplemental Products

Human NLRP3/NALP3 Antibody Summary

Species Reactivity
Detects human NLRP3/NALP3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1, 2, 5, rhNALP3 (aa 540‑689), or recombinant mouse NALP3 is observed.
Monoclonal Mouse IgG1 Clone # 652514
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NLRP3/NALP3
Accession # Q96P20
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NLRP3/NALP3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

NLRP3/NALP3 in Jurkat Human Cell Line. NLRP3/NALP3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP3/NALP3

NLRP3 (NAcht Leucine-rich repeat Protein 3), also known as NALP3, CIAS1, PYPAF or Cryopyrin is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NLRP3 contains an N-terminal Pyrin domain (aa 1-93) followed by a Nacht region (aa 220-536), and seven LRRs (aa 740-991). Within the sequence used as an immunogen, human NLRP3 shares 76% and 75% aa identity with mouse and rat NLRP3, respectively. Isoforms of 979, 979, 922 and 719 aa lack aa 720–776, aa 836-892, both regions, or terminate at aa 719.

Long Name
NLR Family, Pyrin Domain Containing 2
Entrez Gene IDs
114548 (Human); 216799 (Mouse); 287362 (Rat)
Alternate Names
AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1

Product Datasheets

You must select a language.


Citation for Human NLRP3/NALP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mitochondrial NLRP3 protein induces reactive oxygen species to promote Smad protein signaling and fibrosis independent from the inflammasome.
    Authors: Bracey N, Gershkovich B, Chun J, Vilaysane A, Meijndert H, Wright J, Fedak P, Beck P, Muruve D, Duff H
    J Biol Chem, 2014;289(28):19571-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NLRP3/NALP3 Antibody

There are currently no reviews for this product. Be the first to review Human NLRP3/NALP3 Antibody and earn rewards!

Have you used Human NLRP3/NALP3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review