Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 652514
View all NLRP3/NALP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NLRP3/NALP3
Ala4-Val223
Accession # Q96P20

Specificity

Detects human NLRP3/NALP3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1, 2, 5, rhNALP3 (aa 540‑689), or recombinant mouse NALP3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NLRP3/NALP3 Antibody

NLRP3/NALP3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

NLRP3/NALP3 in Jurkat Human Cell Line.

NLRP3/NALP3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human NLRP3/NALP3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NLRP3/NALP3

NLRP3 (NAcht Leucine-rich repeat Protein 3), also known as NALP3, CIAS1, PYPAF or Cryopyrin is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Human NLRP3 contains an N-terminal Pyrin domain (aa 1-93) followed by a Nacht region (aa 220-536), and seven LRRs (aa 740-991). Within the sequence used as an immunogen, human NLRP3 shares 76% and 75% aa identity with mouse and rat NLRP3, respectively. Isoforms of 979, 979, 922 and 719 aa lack aa 720–776, aa 836-892, both regions, or terminate at aa 719.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Gene Symbol

NLRP3

UniProt

Q96P20 (Human)

Additional NLRP3/NALP3 Products

Product Documents for Human NLRP3/NALP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NLRP3/NALP3 Antibody

For research use only

Citations for Human NLRP3/NALP3 Antibody

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FAQs for Human NLRP3/NALP3 Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does this NLRP3/NALP3 antibody come in lyophilized form?

    A: Yes, we carry this NLRP2 antibody in lyophilized format.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: An E. coli derived sequence corresponding to Ala4 - Val223 of human NLRP3/NALP3.

  • Q: Does this NLRP3/NALP3 antibody come in lyophilized form?

    A: Yes, we carry this NLRP2 antibody in lyophilized format.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: An E. coli derived sequence corresponding to Ala4 - Val223 of human NLRP3/NALP3.

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