Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Chlorocebus pygerythrus (Vervet Monkey)

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nogo Receptor/NgR Primary Antibodies »

Product Specifications

Immunogen

recombinant human NgR
Cys27-Ser447
Accession # Q9BZR6

Specificity

Detects human Nogo Receptor (NgR) in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Nogo Receptor/NgR Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG) to immobilized Recombinant Human Nogo R Fc Chimera (Catalog # 1208-NG) coated at 5 µg/mL (100 µL/well). At 100 µg/mL, this antibody will block >90% of the binding.

Western Blot

0.1 µg/mL
Sample: Recombinant Human Nogo Receptor/NgR Fc Chimera (Catalog # 1208-NG)

Reviewed Applications

Read 2 reviews rated 4 using AF1208 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nogo Receptor/NgR

Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTR then sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6).

References

  1. Fournier, A.E. et al. (2001) Nature 409:341.
  2. GrandPre, T. et al. (2002) Nature 417:547.
  3. Wang, K.C. et al. (2002) Nature 420:74.
  4. Barton, W.A. et al. (2003) EMBO Journal 22:3291.
  5. Yamashita, T. and M. Tohyama (2003) Nature Neuroscience 6:461.
  6. Fournier, A.S. et al. (2002) J. Neurosci. 22:8876.

Long Name

Nogo-66 Receptor/Reticulon 4 Receptor

Alternate Names

NgR1, NogoR, RTN4R

Entrez Gene IDs

65078 (Human); 65079 (Mouse)

Gene Symbol

RTN4R

UniProt

Q9BZR6

Additional Nogo Receptor/NgR Products

Product Documents for Human Nogo Receptor/NgR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Nogo Receptor/NgR Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Human Nogo Receptor/NgR Antibody

Customer Reviews for Human Nogo Receptor/NgR Antibody (2)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22281491
    Species: Human
    Verified Customer | Posted 01/05/2015
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 22281491
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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